{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T21:26:49Z","timestamp":1762032409254},"reference-count":49,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,4,1]]},"abstract":"<jats:p>All strains of<jats:italic>Yersinia pestis<\/jats:italic>examined have been found to lack an O-antigen. In other members of the<jats:italic>Enterobacteriaceae<\/jats:italic>, the rough phenotype often results in attenuation. However,<jats:italic>Y. pestis<\/jats:italic>is the aetiological agent of bubonic plague. In evolving from the ancestral enteropathogenic<jats:italic>Yersinia pseudotuberculosis<\/jats:italic>, and with the development of an arthropod-vectored systemic pathogenesis, smooth LPS production is not necessary for<jats:italic>Y. pestis<\/jats:italic>virulence and the metabolic burden has been alleviated by inactivation of the O-antigen biosynthetic operon. To investigate this,<jats:italic>Y. pestis<\/jats:italic>strain KIM D27 was transformed with a plasmid carrying the operon encoding the O-antigen of<jats:italic>Yersinia enterocolitica<\/jats:italic>O : 3. Expression of the O-antigen could be detected in silver-stained gels. The receptor for bacteriophage \u03d5YeO3-12 has been shown to be O-antigen, and infection by this bacteriophage results in lysis of<jats:italic>Y. enterocolitica<\/jats:italic>O : 3. Expression of the O-antigen in<jats:italic>Y. pestis<\/jats:italic>conferred sensitivity to lysis by \u03d5YeO3-12. The O-antigen-expressing clone was shown to be as virulent in mice by the intravenous route of challenge as the rough wild-type. Assays showed no alteration in the ability of<jats:italic>Y. pestis<\/jats:italic>to resist lysis by cationic antimicrobial peptides, serum or polymyxin.<\/jats:p>","DOI":"10.1099\/jmm.0.05044-0","type":"journal-article","created":{"date-parts":[[2003,4,3]],"date-time":"2003-04-03T22:52:16Z","timestamp":1049410336000},"page":"289-294","source":"Crossref","is-referenced-by-count":30,"title":["Expression of heterologous O-antigen in Yersinia pestis KIM does not affect virulence by the intravenous route"],"prefix":"10.1099","volume":"52","author":[{"given":"P.C. 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