{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T02:50:33Z","timestamp":1744080633805},"reference-count":15,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,8,1]]},"abstract":"<jats:p>The potential of human plasma (HP) or serum (HS) as a replacement for fetal calf serum (FCS) was evaluated in a liver infusion tryptose (LIT) medium for bulk cultivation of<jats:italic>Leishmania donovani<\/jats:italic>promastigotes. The promastigote yield with the LIT-FCS standard medium was 0.4\u20131.8\u00d710<jats:sup>7<\/jats:sup>ml<jats:sup>\u22121<\/jats:sup>, and yields of 0.5\u20133.4\u00d710<jats:sup>7<\/jats:sup>(<jats:italic>P<\/jats:italic>\u200a=\u200a0.527) and 0.4\u20132.4\u00d710<jats:sup>7<\/jats:sup>(<jats:italic>P<\/jats:italic>\u200a=\u200a0.062) were recorded for two LIT medium variants containing HP or HS as supplement instead of FCS. Significantly, higher promastigote yields of 1.3\u20134.9\u00d710<jats:sup>7<\/jats:sup>ml<jats:sup>\u22121<\/jats:sup>were demonstrated when LIT medium was supplemented with HP of blood group O but not A, B, AB or equally pooled ABO (<jats:italic>P<\/jats:italic>\u200a=\u200a0.007\u20130.020). Matching (<jats:italic>P<\/jats:italic>\u200a=\u200a0.56) strong positive (1\u200a:\u200a10 2400 to \u22651\u200a:\u200a262 144 00) and weak negative (1\u200a:\u200a5\u20131\u200a:\u200a160) direct agglutination test (DAT) titres, respectively, were demonstrated in 24 visceral leishmaniasis (VL) and 45 non-VL sera for both standard LIT-FCS and alternative LIT-HP derived antigens. Our findings indicate strong potential for sustainable production of promastigotes for important diagnostic procedures such as DAT in the VL affected areas.<\/jats:p>","DOI":"10.1099\/jmm.0.052993-0","type":"journal-article","created":{"date-parts":[[2013,5,9]],"date-time":"2013-05-09T03:59:31Z","timestamp":1368071971000},"page":"1165-1169","source":"Crossref","is-referenced-by-count":4,"title":["Successful substitution of fetal calf serum by human plasma for bulk cultivation of Leishmania donovani promastigotes"],"prefix":"10.1099","volume":"62","author":[{"given":"Abdelhafeiz","family":"Mahamoud","sequence":"first","affiliation":[{"name":"Biomedical Research Laboratory, Ahfad University for Women, PO Box 167, Omdurman, Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hussam Ali","family":"Osman","sequence":"additional","affiliation":[{"name":"Biomedical Research Laboratory, Ahfad University for Women, PO Box 167, Omdurman, Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Durria","family":"Mansour","sequence":"additional","affiliation":[{"name":"Biomedical Research Laboratory, Ahfad University for Women, PO Box 167, Omdurman, Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdallah","family":"el Harith","sequence":"additional","affiliation":[{"name":"Retired microbiologist"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.2307\/3279142"},{"key":"r2","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.1128\/JCM.33.8.1984-1988.1995","article-title":"Evaluation of cleaving agents other than trypsin in direct agglutination test for further improving diagnosis of visceral leishmaniasis","volume":"33","author":"el Harith","year":"1995","journal-title":"J Clin Microbiol"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46753-0"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1007\/BF00925475"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1983.tb00728.x"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(86)90149-5"},{"key":"r7","doi-asserted-by":"crossref","first-page":"962","DOI":"10.4269\/ajtmh.1979.28.962","article-title":"Diagnosis of cutaneous leishmaniasis by in vitro cultivation of saline aspirates in Schneider\u2019s Drosophila Medium","volume":"28","author":"Hendricks","year":"1979","journal-title":"Am J Trop Med Hyg"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(91)90226-O"},{"key":"r9","first-page":"99","article-title":"Requirements of defined cultivation conditions for standard growth of Leishmania promastigotes in vitro","volume":"45","author":"Lemesre","year":"1988","journal-title":"Acta Trop"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/1.1.1"},{"key":"r11","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.4049\/jimmunol.125.5.2195","article-title":"Mechanism of lethal effect of human serum upon Leishmania donovani.","volume":"125","author":"Pearson","year":"1980","journal-title":"J Immunol"},{"key":"r12","doi-asserted-by":"crossref","first-page":"942","DOI":"10.4269\/ajtmh.1986.35.942","article-title":"A new liquid medium without blood and serum for culture of hemoflagellates","volume":"35","author":"Sadigursky","year":"1986","journal-title":"Am J Trop Med Hyg"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.2307\/3277500"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.374-389.2002"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.2307\/3273491"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.052993-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T16:03:30Z","timestamp":1688227410000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.052993-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,1]]},"references-count":15,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2013,8,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.052993-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,1]]}}}