{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T22:48:26Z","timestamp":1695768506327},"reference-count":19,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,1]]},"abstract":"<jats:p>This study was conducted to analyse the presence of a plasmid-mediated carbapenem resistance mechanism in a clinical <jats:italic>Enterobacter aerogenes<\/jats:italic> isolate from a patient from Jiangsu province, People\u2019s Republic of China. PCR and sequencing confirmed that the isolate harboured <jats:italic>Klebsiella pneumoniae<\/jats:italic> carbapenemase (KPC)-2, DHA-1 and TEM-1 \u03b2-lactamase genes. Both the KPC-2 and DHA-1 genes were transferred to <jats:italic>Escherichia coli<\/jats:italic> C600 by transconjugation, and Southern blotting confirmed that these two genes were located on the same plasmid, which was of approximately 56 kb in size. The <jats:italic>Enterobacter aerogenes<\/jats:italic> isolate was resistant to carbapenems and other tested antimicrobial agents. The <jats:italic>Escherichia coli<\/jats:italic> transconjugant showed reduced susceptibility but not resistance to carbapenems and other \u03b2-lactams, indicating the presence of another, possibly permeability-related, resistance mechanism in the clinical isolate.<\/jats:p>","DOI":"10.1099\/jmm.0.054627-0","type":"journal-article","created":{"date-parts":[[2013,10,31]],"date-time":"2013-10-31T03:21:44Z","timestamp":1383189704000},"page":"367-370","source":"Crossref","is-referenced-by-count":9,"title":["KPC-2 carbapenemase and DHA-1 AmpC determinants carried on the same plasmid in Enterobacter aerogenes"],"prefix":"10.1099","volume":"63","author":[{"given":"Shougang","family":"Kuai","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Wuxi Fifth People's Hospital, Wuxi, Jiangsu 214005, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haifeng","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Clinical 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