{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T05:37:19Z","timestamp":1770269839918,"version":"3.49.0"},"reference-count":33,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:p>This study aimed to investigate the genetic association between fluoroquinolone (FQ) and\/or cephalosporin (CEP) resistance in <jats:italic>Escherichia coli<\/jats:italic> isolates from dogs, and the risk to human health. We characterized <jats:italic>E. coli<\/jats:italic> clinical isolates, derived from faecal samples of dogs attending veterinary hospitals, using phylogenetic grouping, determination of virulence factor (VF) prevalence, multilocus sequence typing (MLST) and O serotyping. The D group was the dominant phylogenetic group among strains resistant to FQ and\/or CEP. In contrast, the dominant group among susceptible strains was group B2. Group D strains showed a significantly higher prevalence of VFs than strains belonging to groups A and B1, and were resistant to significantly more antimicrobials than group B2 strains. The phylogenetic distribution of FQ\u2013CEP-resistant <jats:italic>E. coli<\/jats:italic> groups (FQ\u2013CEPRECs) and FQ-resistant groups was significantly correlated (<jats:italic>r<\/jats:italic>\u200a=\u200a0.98), but FQ\u2013CEPRECs and CEP-resistant <jats:italic>E. coli<\/jats:italic> groups were not correlated (<jats:italic>r<\/jats:italic>\u200a=\u200a0.58). Data from PFGE, O serotype and MLST analyses indicated that the majority of FQ-resistant strains derived from a particular lineage of phylogenetic group D: serotype O1 and sequence type (ST) 648. Some D-O1-ST648 strains carried <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CMY-2<\/jats:sub>, showed multidrug resistance and possessed a higher prevalence of the VFs <jats:italic>kspMT<\/jats:italic>, <jats:italic>ompT<\/jats:italic> and PAI compared with other group D strains. Our data indicate that the emergence of FQ-CEP-resistant <jats:italic>E. coli<\/jats:italic> is based primarily on FQ-resistant <jats:italic>E. coli<\/jats:italic>. Moreover, as strains of the D-O1-ST648 lineage have been found in clinical isolates derived from humans at a relatively high frequency, our findings indicate that the spreading of D-O1-ST648 strains may cause serious difficulties in both veterinary and human clinical fields in the future.<\/jats:p>","DOI":"10.1099\/jmm.0.054676-0","type":"journal-article","created":{"date-parts":[[2013,2,22]],"date-time":"2013-02-22T04:30:52Z","timestamp":1361507452000},"page":"263-270","source":"Crossref","is-referenced-by-count":11,"title":["Phylogenetic association of fluoroquinolone and cephalosporin resistance of D-O1-ST648 Escherichia coli carrying bla\n            CMY-2 from faecal samples of dogs in Japan"],"prefix":"10.1099","volume":"63","author":[{"given":"Toyotaka","family":"Sato","sequence":"first","affiliation":[{"name":"Laboratory of Food Microbiology and Food Safety, Department of Health and Environmental Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-ichi","family":"Yokota","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torahiko","family":"Okubo","sequence":"additional","affiliation":[{"name":"Laboratory of Food Microbiology and Food Safety, Department of Health and Environmental Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Usui","sequence":"additional","affiliation":[{"name":"Laboratory of Food Microbiology and Food Safety, Department of Health and Environmental Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhiro","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Tamura","sequence":"additional","affiliation":[{"name":"Laboratory of Food Microbiology and Food Safety, Department of Health and Environmental Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01480-06"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00856-08"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.08.007"},{"key":"r5","article-title":"Performance Standards for Antimicrobial Disk and Dilution Antimicrobial Susceptibility Tests for Bacteria Isolated from Animals; 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