{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T22:44:36Z","timestamp":1784933076211,"version":"3.55.0"},"reference-count":47,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,1]]},"abstract":"<jats:p>The co-existence of carbapenemase, 16S rRNA methylase and mutated quinolone resistance-determining regions (QRDRs) can cause serious difficulty in treating infections with multidrug-resistant <jats:italic>Acinetobacter baumannii.<\/jats:italic> In this study, we aimed to determine the mechanisms of imipenem, amikacin and ciprofloxacin resistance in <jats:italic>A. baumannii<\/jats:italic> isolates with resistance to these antibiotics. A total of 31 non-duplicate isolates of amikacin- and ciprofloxacin-resistant <jats:italic>Acinetobacter<\/jats:italic> isolates were identified from April to August 2010 from a single hospital in South Korea. To assess the clonal relatedness of the 31 <jats:italic>Acinetobacter<\/jats:italic> isolates, multilocus sequence typing, network phylogenetic analysis and enterobacterial repetitive intergenic consensus-PCR were utilized. Detection of OXA-type carbapenemase and 16S rRNA methylase was conducted using a multiplex PCR assay. The QRDRs of the <jats:italic>gyrA<\/jats:italic> and <jats:italic>parC<\/jats:italic> genes were amplified and sequenced. The result showed that 30\/31 isolates harboured the <jats:italic>bla<\/jats:italic>\n                  <jats:sub>OXA-23<\/jats:sub>-like carbapenemase, which made them resistant to imipenem (MICs \u226516 \u00b5g ml<jats:sup>\u22121<\/jats:sup>). Twenty-eight of the 31 isolates were found to possess <jats:italic>armA<\/jats:italic>, a 16S rRNA methylase gene, and showed resistance to amikacin, arbekacin, gentamicin and tobramycin (MICs &gt;256 \u00b5g ml<jats:sup>\u22121<\/jats:sup>). All of the isolates were determined to carry QRDR mutations in both <jats:italic>gyrA<\/jats:italic> and <jats:italic>parC<\/jats:italic>: a Ser83Leu substitution in <jats:italic>gyrA<\/jats:italic> and a Ser80Leu substitution in <jats:italic>parC<\/jats:italic>, causing a ciprofloxacin MIC \u226564 \u00b5g ml<jats:sup>\u22121<\/jats:sup>. In conclusion, <jats:italic>A. baumannii<\/jats:italic> with co-existence of carbapenemase, 16S rRNA methylase and mutated QRDRs are extremely prevalent in South Korea, which may cause serious problems in the treatment of <jats:italic>A. baumannii<\/jats:italic> infections using carbapenem, amikacin and ciprofloxacin.<\/jats:p>","DOI":"10.1099\/jmm.0.055384-0","type":"journal-article","created":{"date-parts":[[2013,3,22]],"date-time":"2013-03-22T04:38:31Z","timestamp":1363927111000},"page":"836-844","source":"Crossref","is-referenced-by-count":14,"title":["Co-existence of bla\n            OXA-23 and armA in multidrug-resistant Acinetobacter baumannii isolated from a hospital in South Korea"],"prefix":"10.1099","volume":"62","author":[{"given":"Seung Bok","family":"Hong","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Science, Chungbuk Health & Science University, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyeong 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