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We have profiled bacterial consortia sampled from the palatine tonsil and posterior pharyngeal wall microbiotas of healthy adult volunteers (<jats:italic>n<\/jats:italic>\u200a=\u200a10) using differential culture and 16S rRNA gene sequencing, together with PCR-denaturing gradient gel electrophoresis. The data generated were used to assess the validity of an oropharyngeal microcosm system based on replicated constant-depth film fermenters (CDFFs;<jats:italic>n<\/jats:italic>\u200a=\u200a5), which were continuously fed using an artificial airway surface liquid. Developed microcosms exhibited significant homology to<jats:italic>ex situ<\/jats:italic>consortia according to principal components analysis, whilst compositional reproducibility was apparent in replicated models for tonsillar and pharyngeal inocula. Differential viable count data and Shannon\u2013Weiner diversity indices indicated that representative tonsil and pharyngeal model systems achieved dynamic compositional stability about 6 days after inoculation which could be maintained for \u226520 days. In conclusion, the CDFF facilitated the continuous maintenance of bacteriologically stable microcosms that were compositionally similar to<jats:italic>ex situ<\/jats:italic>inocula.<\/jats:p>","DOI":"10.1099\/jmm.0.055806-0","type":"journal-article","created":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T18:06:30Z","timestamp":1362161190000},"page":"906-916","source":"Crossref","is-referenced-by-count":11,"title":["Continuous culture of sessile human oropharyngeal microbiotas"],"prefix":"10.1099","volume":"62","author":[{"given":"Gavin J.","family":"Humphreys","sequence":"first","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences, The University of Manchester, Manchester M13 9PT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"McBain","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences, The University of Manchester, Manchester M13 9PT, 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