{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T13:00:38Z","timestamp":1761310838644},"reference-count":26,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,5,1]]},"abstract":"<jats:p>Shared strains of <jats:italic>Pseudomonas aeruginosa<\/jats:italic> are now well recognized in people with cystic fibrosis (CF), and suitable <jats:italic>P. aeruginosa<\/jats:italic> laboratory typing tools are pivotal to understanding their clinical significance and guiding infection control policies in CF clinics. We therefore compared a single-nucleotide polymorphism (SNP)-based typing method using Sequenom iPLEX matrix-assisted laser desorption ionization with time-of-flight mass spectrometry (MALDI-TOF MS) with typing methods used routinely by our laboratory. We analysed 617 <jats:italic>P. aeruginosa<\/jats:italic> isolates that included 561 isolates from CF patients collected between 2001 and 2009 in two Brisbane CF clinics and typed previously by enterobacterial repetitive intergenic consensus (ERIC)-PCR, as well as 56 isolates from non-CF patients analysed previously by multilocus sequence typing (MLST). The isolates were tested using a <jats:italic>P. aeruginosa<\/jats:italic> Sequenom iPLEX MALDI-TOF (PA iPLEX) method comprising two multiplex reactions, a 13-plex and an 8-plex, to characterize 20 SNPs from the <jats:italic>P. aeruginosa<\/jats:italic> housekeeping genes <jats:italic>acsA<\/jats:italic>, <jats:italic>aroE<\/jats:italic>, <jats:italic>guaA<\/jats:italic>, <jats:italic>mutL<\/jats:italic>, <jats:italic>nuoD<\/jats:italic>, <jats:italic>ppsA<\/jats:italic> and <jats:italic>trpE<\/jats:italic>. These 20 SNPs were employed previously in a real-time format involving 20 separate assays in our laboratory. The SNP analysis revealed 121 different SNP profiles for the 561 CF isolates. Overall, there was at least 96\u200a% agreement between the ERIC-PCR and SNP analyses for all predominant shared strains among patients attending our CF clinics: AUST-01, AUST-02 and AUST-06. For the less frequently encountered shared strain AUST-07, 6\/25 (24\u200a%) ERIC-PCR profiles were misidentified initially as AUST-02 or as unique, illustrating the difficulty of gel-based analyses. SNP results for the 56 non-CF isolates were consistent with previous MLST data. Thus, the PA iPLEX format provides an attractive high-throughput alternative to ERIC-PCR for large-scale investigations of shared <jats:italic>P. aeruginosa<\/jats:italic> strains.<\/jats:p>","DOI":"10.1099\/jmm.0.055905-0","type":"journal-article","created":{"date-parts":[[2013,2,15]],"date-time":"2013-02-15T04:17:39Z","timestamp":1360901859000},"page":"734-740","source":"Crossref","is-referenced-by-count":9,"title":["High-throughput single-nucleotide polymorphism-based typing of shared Pseudomonas aeruginosa strains in cystic fibrosis patients using the Sequenom iPLEX platform"],"prefix":"10.1099","volume":"62","author":[{"given":"Melanie W.","family":"Syrmis","sequence":"first","affiliation":[{"name":"Queensland Children\u2019s Medical Research Institute, Royal Children\u2019s Hospital, The University of Queensland, Brisbane, Queensland, Australia"},{"name":"Queensland Paediatric Infectious Diseases Laboratory, Royal Children\u2019s Hospital, Brisbane, Queensland, 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