{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:51:20Z","timestamp":1790531480205,"version":"4.1.0"},"reference-count":46,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,12,1]]},"abstract":"<jats:p>\n                  <jats:italic>Listeria monocytogenes<\/jats:italic> is an important food-borne bacterial pathogen and listeriosis can result in abortions in pregnant women. The bacterium can colonize food-processing environments, where specific molecular subtypes can persist for years. The purpose of this study was to determine the virulence potential of a group of food-processing persistent <jats:italic>L. monocytogenes<\/jats:italic> strains encoding a premature stop codon in <jats:italic>inlA<\/jats:italic> (encoding internalin A) by using two orally dosed models, pregnant mice and pregnant guinea pigs. A food-processing persistent strain of <jats:italic>L. monocytogenes<\/jats:italic> invaded placentas (<jats:italic>n<\/jats:italic>\u200a=\u200a58; 10\u200a% positive) and fetuses (3\u200a% positive) of pregnant mice (<jats:italic>n<\/jats:italic>\u200a=\u200a9 animals per strain), similar to a genetically manipulated murinized strain, EGD-e InlA<jats:italic>\n                     <jats:sup>m*<\/jats:sup>\n                  <\/jats:italic> (<jats:italic>n<\/jats:italic>\u200a=\u200a61; 3 and 2\u200a%, respectively). In pregnant guinea pigs (<jats:italic>n<\/jats:italic>\u200a=\u200a9 animals per bacterial strain), a maternofetal strain (from a human fetal clinical fatal case) was isolated from 34\u200a% of placenta samples (<jats:italic>n<\/jats:italic>\u200a=\u200a50), whereas both food-processing persistent strains were found in 5\u200a% of placenta samples (<jats:italic>n<\/jats:italic>\u200a=\u200a36 or 37). One of the food-processing persistent strains, N53-1, was found in up to 8\u200a% of guinea pig fetal liver and brain samples, whereas the maternofetal control was found in 6\u200a% of fetal tissue samples. As the food-processing persistent strains carry a premature stop codon in <jats:italic>inlA<\/jats:italic> but are invasive in orally dosed pregnant mice and guinea pigs, we hypothesize that listerial crossing of the placental barrier can occur by a mechanism that is independent of an interaction between E-cadherin and InlA.<\/jats:p>","DOI":"10.1099\/jmm.0.057505-0","type":"journal-article","created":{"date-parts":[[2013,9,7]],"date-time":"2013-09-07T05:27:29Z","timestamp":1378531649000},"page":"1799-1806","source":"Crossref","is-referenced-by-count":25,"title":["Listeria monocytogenes strains encoding premature stop codons in inlA invade mice and guinea pig fetuses in orally dosed dams"],"prefix":"10.1099","volume":"62","author":[{"given":"Anne","family":"Holch","sequence":"first","affiliation":[{"name":"National Food Institute, Technical University of Denmark, S\u00f8ltofts Plads, Bldg 221, DK-2800 Kongens Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne","family":"Ingmer","sequence":"additional","affiliation":[{"name":"Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigb\u00f8jlen 4, DK-1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tine Rask","family":"Licht","sequence":"additional","affiliation":[{"name":"National Food Institute, Technical University of Denmark, M\u00f8rkh\u00f8j Bygade 19, DK-2860 S\u00f8borg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lone","family":"Gram","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Technical University of Denmark, S\u00f8ltofts Plads, Bldg 221, DK-2800 Kongens Lyngby, 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