{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T15:13:17Z","timestamp":1790176397749,"version":"4.1.0"},"reference-count":44,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,7,1]]},"abstract":"<jats:p>Cervical cancer is a human papilloma virus (HPV)-related cancer, but most HPV infections are transient or intermittent and resolve spontaneously. Thus, other factors, such as cervical microflora, which are dominated by lactobacilli, must be involved in invasive cervical carcinoma development after HPV infection. Previous studies have demonstrated that lactobacilli have antitumour effects, and it is possible that vaginal lactobacilli prevent cervical cancer. Here we examined the proliferative and apoptotic responses of normal and tumour cervical cells to common vaginal lactobacilli components by investigating human normal fibroblast-like cervical (normal cervical) and HeLa (cervical tumour) cell responses to <jats:italic>Lactobacillus gasseri<\/jats:italic> and <jats:italic>Lactobacillus crispatus<\/jats:italic>. The effects of different lactobacilli components, such as culture supernatants, cytoplasmic extracts, cell-wall extracts and live cells, were determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, trypan blue staining, lactate dehydrogenase assay and colorimetric caspase-3 activity assay. Changes in caspase-3 and human chorionic gonadotropin \u03b2 (hCG\u03b2) expression were analysed by quantitative RT-PCR. Tumour cell growth inhibition by culture supernatants was higher than that by pH- and lactate-adjusted controls. However, the effects of the supernatants on normal cells were similar to those of lactate-adjusted controls. Apoptosis was inhibited by supernatants, which was consistent with higher hCG\u03b2 expression since hCG inhibits apoptosis. Our study demonstrated that common vaginal lactobacilli exert cytotoxic effects on cervical tumour cells, but not on normal cells, and that this cytotoxicity is independent of pH and lactate. Our results encourage further studies on the interaction between lactobacilli and cervical cells, and administration of common vaginal lactobacilli as probiotics.<\/jats:p>","DOI":"10.1099\/jmm.0.057521-0","type":"journal-article","created":{"date-parts":[[2013,4,26]],"date-time":"2013-04-26T01:49:11Z","timestamp":1366940951000},"page":"1065-1072","source":"Crossref","is-referenced-by-count":112,"title":["Normal and tumour cervical cells respond differently to vaginal lactobacilli, independent of pH and lactate"],"prefix":"10.1099","volume":"62","author":[{"given":"Elahe","family":"Motevaseli","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahdieh","family":"Shirzad","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Mohammad","family":"Akrami","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azam-Sadat","family":"Mousavi","sequence":"additional","affiliation":[{"name":"Department of Gynecology Oncology, Valli-e-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akbar","family":"Mirsalehian","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Hossein","family":"Modarressi","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","first-page":"1992","article-title":"The 2001 Bethesda System terminology","volume":"68","author":"Apgar","year":"2003","journal-title":"Am Fam Physician"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgq176"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.4.244"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.2002.6655"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2006.01913.x"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2004.07.020"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-0125"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(86)90368-6"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06262-11"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-11-1-177"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(01)00496-7"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1177\/1756283X12459294"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2006.02.019"},{"key":"r14","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1093\/ajcn\/73.6.1142S","article-title":"Probiotics in human disease","volume":"73","author":"Isolauri","year":"2001","journal-title":"Am J Clin Nutr"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01137.x"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1438.2007.01151.x"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-7-26"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00791.x"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00131.2010"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.12.053"},{"key":"r21","first-page":"123","article-title":"Cell-bound exopolysaccharide from probiotic bacteria induces autophagic cell death of tumour cells","volume":"51","author":"Kim","year":"2010","journal-title":"Lett Appl Microbiol"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-006-0010-3"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.04.131"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1177\/1933719111418379"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00772.x"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1157\/13116753"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.3802\/jgo.2009.20.1.39"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3182267ac4"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1080\/08923970802443631"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2012.717676"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.t01-1-00275.x"},{"key":"r32","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-13-43"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2009.06.027"},{"key":"r34","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002611107"},{"key":"r35","doi-asserted-by":"publisher","DOI":"10.1080\/01635580701365084"},{"key":"r36","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2005.10.007"},{"key":"r37","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.18.148"},{"key":"r38","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2010.11.041"},{"key":"r39","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.11.36"},{"key":"r40","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-7-128"},{"key":"r41","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2746-2749.2002"},{"key":"r42","first-page":"281","article-title":"[Analysis of diversity of vaginal microbiota in healthy Chinese women by using DNA-fingerprinting]","volume":"44","author":"Xiao","year":"2012","journal-title":"Beijing Da Xue Xue Bao"},{"key":"r43","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207050200"},{"key":"r44","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00851-09"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.057521-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T03:16:14Z","timestamp":1601608574000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.057521-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,1]]},"references-count":44,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.057521-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,1]]}}}