{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T17:17:40Z","timestamp":1776446260098,"version":"3.51.2"},"reference-count":9,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,10,1]]},"abstract":"<jats:p>A universal stool extraction method for recovery of nucleic acids (NAs) from gastrointestinal pathogens was developed to support rapid diagnostics for the London 2012 Olympics. The method involved mechanical disruption (bead beating) of the stools, followed by automated extraction and detection using real-time PCR. This method had been used extensively in the Second Infectious Intestinal Disease Study (IID2) for the isolation of NA from bacteria and parasites (and was effective for the robust recovery of <jats:italic>Cryptosporidium<\/jats:italic> spp.) but had not been used for enteric viruses. To ensure this method was universally suitable, panels of samples known to contain target bacteria, viruses or parasites were processed in triplicate using the pre-treatment method routinely used for each target and the new extraction method (bead beating). The extracts were tested using real-time PCR and the cycle threshold values were compared. The results from this study showed that bead beating improved yields for the bacterial and parasitic targets and was suitable for the viral targets. The implementation of this universal method should confer cost- and time-saving benefits and streamline the processes required for the characterization of an array of pathogens from faecal samples.<\/jats:p>","DOI":"10.1099\/jmm.0.058743-0","type":"journal-article","created":{"date-parts":[[2013,7,6]],"date-time":"2013-07-06T04:07:03Z","timestamp":1373083623000},"page":"1535-1539","source":"Crossref","is-referenced-by-count":22,"title":["Universal extraction method for gastrointestinal pathogens"],"prefix":"10.1099","volume":"62","author":[{"given":"Fenella D.","family":"Halstead","sequence":"first","affiliation":[{"name":"PHE Microbiology Services, Colindale, London, UK"},{"name":"Royal Free London NHS Trust, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adele V.","family":"Lee","sequence":"additional","affiliation":[{"name":"Burnet Institute, Melbourne, Australia"},{"name":"PHE Public Health Laboratory London, Barts Health NHS Trust, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xose","family":"Couto-Parada","sequence":"additional","affiliation":[{"name":"PHE Public Health Laboratory London, Barts Health NHS Trust, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Spencer D.","family":"Polley","sequence":"additional","affiliation":[{"name":"University College London Hospitals NHS Trust, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clare","family":"Ling","sequence":"additional","affiliation":[{"name":"PHE Public Health Laboratory London, Barts Health NHS Trust, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Jenkins","sequence":"additional","affiliation":[{"name":"PHE Microbiology Services, Colindale, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel M.","family":"Chalmers","sequence":"additional","affiliation":[{"name":"Cryptosporidium Reference Laboratory, Public Health Wales Microbiology, Swansea, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristin","family":"Elwin","sequence":"additional","affiliation":[{"name":"Cryptosporidium Reference Laboratory, Public Health Wales Microbiology, Swansea, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jim J.","family":"Gray","sequence":"additional","affiliation":[{"name":"Norfolk and Norwich University Hospitals NHS Trust, UK"},{"name":"PHE Microbiology Services, Colindale, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miren","family":"Iturriza-G\u00f3mara","sequence":"additional","affiliation":[{"name":"PHE Microbiology Services, Colindale, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Wain","sequence":"additional","affiliation":[{"name":"PHE Microbiology Services, Colindale, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duncan A.","family":"Clark","sequence":"additional","affiliation":[{"name":"PHE Public Health Laboratory London, Barts Health NHS Trust, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick J.","family":"Bolton","sequence":"additional","affiliation":[{"name":"PHE Public Health Laboratory, Manchester, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohini J.","family":"Manuel","sequence":"additional","affiliation":[{"name":"PHE Public Health Laboratory London, Barts Health NHS Trust, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"the Olympics GI Group","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/JCM.28.3.495-503.1990","article-title":"Rapid and simple method for purification of nucleic acids","volume":"28","author":"Boom","year":"1990","journal-title":"J Clin Microbiol"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2013.03.001"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2012.02.006"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.19.1.165-256.2006"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1136\/jech.57.2.102"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.08.016"},{"key":"r8","doi-asserted-by":"crossref","first-page":"3153","DOI":"10.1128\/JCM.37.10.3153-3158.1999","article-title":"Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis","volume":"37","author":"McLauchlin","year":"1999","journal-title":"J Clin Microbiol"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2288-10-39"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2011.238386"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.058743-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T03:17:29Z","timestamp":1601608649000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.058743-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,1]]},"references-count":9,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2013,10,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.058743-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,1]]}}}