{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T14:44:57Z","timestamp":1774795497867,"version":"3.50.1"},"reference-count":28,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,10,1]]},"abstract":"<jats:p>Antimicrobial susceptibility, integron carriage, genetic relationship and presence of some important virulence genes of the integron-carrier strains of<jats:italic>Shigella sonnei<\/jats:italic>(<jats:italic>n<\/jats:italic>\u200a=\u200a230) and<jats:italic>Shigella flexneri<\/jats:italic>(<jats:italic>n<\/jats:italic>\u200a=\u200a22) isolated from stool samples of patients in Hungary between 1998 and 2008 were investigated. Sixty-seven per cent (168\/252) of the strains were resistant to sulfamethoxazole\/trimethoprim (SxT) followed by streptomycin (S, 47\u200a%), ampicillin (A, 32\u200a%) and tetracycline (Tc, 28\u200a%). Thirty-six per cent (90\/252) exhibited multidrug resistance, mostly showing SSxTTc or ASSxTc, ASSxTTc resistance patterns. An<jats:italic>S<\/jats:italic>.<jats:italic>sonnei<\/jats:italic>strain of imported origin was resistant to cefotaxime and harboured a<jats:italic>bla<\/jats:italic><jats:sub>CTX-M-55<\/jats:sub>-type extended-spectrum \u03b2-lactamase gene. Altogether 33\u200a% of the<jats:italic>S. sonnei<\/jats:italic>(<jats:italic>n<\/jats:italic>\u200a=\u200a75) and 14\u200a% of the<jats:italic>S. flexneri<\/jats:italic>(<jats:italic>n<\/jats:italic>\u200a=\u200a3) strains had either class 1 or class 2 integrons or both. The variable regions encoded<jats:italic>aadA1<\/jats:italic>or<jats:italic>dfrA1-aadA1<\/jats:italic>genes for the class 1 and<jats:italic>dfrA1-sat2-aadA1<\/jats:italic>or<jats:italic>dfrA1-sat2<\/jats:italic>genes for the class 2 integrons. Pulsed-field gel electrophoresis analysis revealed that those strains that have different integron types represented different genetic clusters. The Shiga toxin (<jats:italic>stx1<\/jats:italic>) gene was identified in one<jats:italic>S. sonnei<\/jats:italic>strain and the<jats:italic>cdtB<\/jats:italic>gene was detected in an<jats:italic>S. flexneri<\/jats:italic>strain. The results reveal the high incidence of antibiotic resistance among<jats:italic>Shigella<\/jats:italic>isolates and the presence of the<jats:italic>stx1<\/jats:italic>gene in<jats:italic>S. sonnei<\/jats:italic>and the<jats:italic>cdtB<\/jats:italic>gene in<jats:italic>S. flexneri<\/jats:italic>. The genetic diversity of<jats:italic>Shigella<\/jats:italic>spp. isolated recently in Hungary was also demonstrated.<\/jats:p>","DOI":"10.1099\/jmm.0.058917-0","type":"journal-article","created":{"date-parts":[[2013,6,26]],"date-time":"2013-06-26T03:24:44Z","timestamp":1372217084000},"page":"1545-1551","source":"Crossref","is-referenced-by-count":19,"title":["Antimicrobial resistance and genetic characteristics of integron-carrier shigellae isolated in Hungary (1998\u20132008)"],"prefix":"10.1099","volume":"62","author":[{"given":"No\u00e9mi","family":"N\u00f3gr\u00e1dy","sequence":"first","affiliation":[{"name":"Department of Phage Typing and Molecular Epidemiology, National Center for Epidemiology, H-1097 Budapest, Gy\u00e1li \u00fat 2-6, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margit","family":"Kir\u00e1ly","sequence":"additional","affiliation":[{"name":"Department of Phage Typing and Molecular Epidemiology, National Center for Epidemiology, H-1097 Budapest, Gy\u00e1li \u00fat 2-6, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kl\u00e1ra","family":"Borb\u00e1s","sequence":"additional","affiliation":[{"name":"Central Regional Laboratory of Enteric Pathogens, National Center for Epidemiology, H-1097 Budapest, Gy\u00e1li \u00fat 2-6, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1kos","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, National Center for Epidemiology, H-1097 Budapest, Gy\u00e1li \u00fat 2-6, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judit","family":"P\u00e1szti","sequence":"additional","affiliation":[{"name":"Department of Phage Typing and Molecular Epidemiology, National Center for Epidemiology, H-1097 Budapest, Gy\u00e1li \u00fat 2-6, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Istv\u00e1n","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, H-1143, Budapest, Hung\u00e1ria krt. 21, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.7883\/yoken.JJID.2008.135","article-title":"CTX-M-3 type \u03b2-lactamase producing Shigella sonnei isolates from pediatric bacillary dysentery cases","volume":"61","author":"Acikgoz","year":"2008","journal-title":"Jpn J Infect Dis"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46725-0"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/151.5.766"},{"key":"r4","doi-asserted-by":"crossref","first-page":"3462","DOI":"10.1128\/AEM.62.9.3462-3465.1996","article-title":"Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes","volume":"62","author":"China","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"r5","article-title":"Performance Standards for Antimicrobial Susceptibility Testing; Approved Standard, 9th edn, 15th Informational Supplement M100\u2013S15","year":"2005"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn287"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.4.1269-1276.2001"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1089\/107662903322541856"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1987.tb02548.x"},{"key":"r12","first-page":"107","article-title":"Antimicrobial susceptibility of Shigella isolates in eight Asian countries, 2001\u20132004","volume":"41","author":"Kuo","year":"2008","journal-title":"J Microbiol Immunol Infect"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.1.185"},{"key":"r14","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1111\/j.1348-0421.1993.tb01675.x","article-title":"Detection of various variant verotoxin genes in Escherichia coli by polymerase chain reaction","volume":"37","author":"Lin","year":"1993","journal-title":"Microbiol Immunol"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01669.x"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm340"},{"key":"r18","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1128\/CMR.11.1.142","article-title":"Diarrheagenic Escherichia coli","volume":"11","author":"Nataro","year":"1998","journal-title":"Clin Microbiol Rev"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2005.04.015"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1080\/03079450600827007"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.421-423.2003"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1016\/S0882-4010(95)90022-5"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl228"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki012"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2006.3.59"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1347-1351.2006"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7588-7595.2001"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4285-4291.2003"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl506"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.9.2658-2661.2001"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2007.07.017"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.058917-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,2]],"date-time":"2023-07-02T16:46:47Z","timestamp":1688316407000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.058917-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,1]]},"references-count":28,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2013,10,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.058917-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,1]]}}}