{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:51:16Z","timestamp":1761519076526},"reference-count":26,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:p>The aims of this study were to (1) conduct a national survey of<jats:italic>Neisseria gonorrhoeae<\/jats:italic>identification by National Neisseria Network (NNN) reference laboratories contributing data to the Australian Gonococcal Surveillance Programme and (2) determine the prevalence in Australia of strains of<jats:italic>N. gonorrhoeae<\/jats:italic>lacking gene sequences commonly targeted by in-house PCR assays for confirmation of gonococcal nucleic acid amplification tests. Gonococcal clinical isolates referred to NNN laboratories for the first half of 2012 were screened using in-house real-time PCR assays targeting multicopy<jats:italic>opa<\/jats:italic>,<jats:italic>porA<\/jats:italic>pseudogene and<jats:italic>cppB<\/jats:italic>genes. There were 2455 clinical gonococcal isolates received in the study period; 98.6\u200a% (2420\/2455) of isolates harboured all three gene targets, 0.12\u200a% (3\/2455) were<jats:italic>porA<\/jats:italic>-negative, 0.04\u200a% (1\/2455)<jats:italic>opa<\/jats:italic>-negative and 1.14\u200a% (28\/2455)<jats:italic>cppB<\/jats:italic>-negative by PCR. Notably, no isolates were simultaneously negative for two targets. However, three isolates failed to be amplified by all three PCR methods, one isolate of which was shown to be a commensal<jats:italic>Neisseria<\/jats:italic>strain by 16S rRNA sequencing. Using PCR as the reference standard the results showed that (1) identification of<jats:italic>N. gonorrhoeae<\/jats:italic>isolates by NNN laboratories was highly specific (99.96\u200a%) and (2) strains of<jats:italic>N. gonorrhoeae<\/jats:italic>lacking gene sequences commonly targeted by in-house PCR assays are present but not widespread throughout Australia at this point in time.<\/jats:p>","DOI":"10.1099\/jmm.0.065094-0","type":"journal-article","created":{"date-parts":[[2013,10,4]],"date-time":"2013-10-04T00:18:29Z","timestamp":1380845909000},"page":"45-49","source":"Crossref","is-referenced-by-count":12,"title":["A national quality assurance survey of Neisseria gonorrhoeae testing"],"prefix":"10.1099","volume":"63","author":[{"given":"Ella","family":"Trembizki","sequence":"first","affiliation":[{"name":"Queensland Children\u2019s Medical Research Institute, University of Queensland, Queensland, Australia"},{"name":"Queensland Paediatric Infectious Diseases Laboratory, Queensland Children\u2019s Health Services, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Lahra","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerrie","family":"Stevens","sequence":"additional","affiliation":[{"name":"Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Freeman","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiffany","family":"Hogan","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geoff","family":"Hogg","sequence":"additional","affiliation":[{"name":"Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Lawrence","sequence":"additional","affiliation":[{"name":"Microbiology and Infectious Diseases Department, Women's and Children's Hospital, North Adelaide, South Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Athena","family":"Limnios","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Pearson","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine-WA, Royal Perth Hospital, Perth, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Smith","sequence":"additional","affiliation":[{"name":"Public Health Microbiology, Communicable Disease, Queensland Health Forensic and Scientific Services, Archerfield, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Nissen","sequence":"additional","affiliation":[{"name":"Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women\u2019s Hospital Campus, Queensland, Australia"},{"name":"Queensland Children\u2019s Medical Research Institute, University of Queensland, Queensland, Australia"},{"name":"Queensland Paediatric Infectious Diseases Laboratory, Queensland Children\u2019s Health Services, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theo","family":"Sloots","sequence":"additional","affiliation":[{"name":"Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women\u2019s Hospital Campus, Queensland, Australia"},{"name":"Queensland Children\u2019s Medical Research Institute, University of Queensland, Queensland, Australia"},{"name":"Queensland Paediatric Infectious Diseases Laboratory, Queensland Children\u2019s Health Services, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Whiley","sequence":"additional","affiliation":[{"name":"Queensland Children\u2019s Medical Research Institute, University of Queensland, Queensland, Australia"},{"name":"Queensland Paediatric Infectious Diseases Laboratory, Queensland Children\u2019s Health Services, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"on behalf of \u2018GRAND\u2019 study investigators","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46108-0"},{"year":"2012","key":"r2","article-title":"Annual Report of the Australian Gonococcal Surveillance Programme, 2011"},{"year":"2012","key":"r3"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-5-35"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.9.4332-4334.2004"},{"key":"r6","doi-asserted-by":"crossref","first-page":"20101","DOI":"10.2807\/ese.17.09.20101-en","article-title":"Identification of Neisseria gonorrhoeae isolates with a recombinant porA gene in Scotland, United Kingdom, 2010 to 2011","volume":"17","author":"Eastick","year":"2012","journal-title":"Eur Surveill"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-005-1319-5"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5653-5659.2005"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.12.007"},{"key":"r10","doi-asserted-by":"crossref","first-page":"20102","DOI":"10.2807\/ese.17.09.20102-en","article-title":"Clinical Neisseria gonorrhoeae isolate with a N. meningitidis porA gene and no prolyliminopeptidase activity, Sweden, 2011: danger of false-negative genetic and culture diagnostic results","volume":"17","author":"Golparian","year":"2012","journal-title":"Eur Surveill"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.45.5.439"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-050829"},{"key":"r12a","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-050829"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00385-09"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2004.013805"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02233-10"},{"key":"r16","first-page":"358","article-title":"Guidelines for the use and interpretation of nucleic acid detection tests for Neisseria gonorrhoeae in Australia: a position paper on behalf of the Public Health Laboratory Network","volume":"29","author":"Smith","year":"2005","journal-title":"Commun Dis Intell Q Rep"},{"key":"r17","first-page":"8","article-title":"Use of a quality assurance scheme in a long-term multicentric study of antibiotic susceptibility of Neisseria gonorrhoeae","volume":"66","author":"Tapsall","year":"1990","journal-title":"Genitourin Med"},{"volume-title":"Antimicrobial Resistance in Neisseria gonorrhoeae","year":"2001","author":"Tapsall","key":"r18"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01108-09"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2006.021733"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp098"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-007-0993-x"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.050045"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1071\/SH07050"},{"key":"r25","doi-asserted-by":"crossref","first-page":"19874","DOI":"10.2807\/ese.16.21.19874-en","article-title":"False-negative results using Neisseria gonorrhoeae porA pseudogene PCR \u2013 a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011","volume":"16","author":"Whiley","year":"2011","journal-title":"Eur Surveill"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.065094-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T21:27:14Z","timestamp":1646688434000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.065094-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,1]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.065094-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"type":"print","value":"0022-2615"},{"type":"electronic","value":"1473-5644"}],"subject":[],"published":{"date-parts":[[2014,1,1]]}}}