{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T03:58:55Z","timestamp":1777521535367,"version":"3.51.4"},"reference-count":59,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:p>The activity of moxifloxacin was compared with ofloxacin and doxycycline against bacteria associated with periodontitis within a biofilm (single strain and mixed population) <jats:italic>in vitro<\/jats:italic>. MICs and minimal bactericidal concentrations (MBCs) of moxifloxacin, ofloxacin and doxycyline were determined against single strains and mixed populations in a planktonic state. Single-species biofilms of two <jats:italic>Porphyromonas gingivalis<\/jats:italic> and two <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic> strains and a multispecies biofilm consisting of 12 species were formed for 3 days. The minimal biofilm eradication concentrations (MBECs) were determined after exposing the biofilms to the antibacterials (0.002\u2013512 \u00b5g ml<jats:sup>\u22121<\/jats:sup>) for 18 h, addition of nutrient broth for 3 days and subsequent subcultivation. Photographs were taken using confocal laser-scanning microscopy and scanning electron microscopy. The MICs and MBCs did not differ between ofloxacin and moxifloxacin against <jats:italic>A. actinomycetemcomitans<\/jats:italic>, whilst moxifloxacin was more active than the other tested antibacterials against anaerobes and the mixed population. The single-species biofilms were eradicated by moderate concentrations of the antibacterials, and the lowest MBECs were always found for moxifloxacin (2\u20138 \u00b5g ml<jats:sup>\u22121<\/jats:sup>). MBECs against the multispecies biofilms were 128, &gt;512 and &gt;512 \u00b5g ml<jats:sup>\u22121<\/jats:sup> for moxifloxacin, ofloxacin and doxycycline, respectively. In summary, moxifloxacin in a topical formulation may have potential as an adjunct to mechanical removal of the biofilms.<\/jats:p>","DOI":"10.1099\/jmm.0.065441-0","type":"journal-article","created":{"date-parts":[[2013,11,12]],"date-time":"2013-11-12T02:35:31Z","timestamp":1384223731000},"page":"284-292","source":"Crossref","is-referenced-by-count":24,"title":["Antibacterial activity of moxifloxacin on bacteria associated with periodontitis within a biofilm"],"prefix":"10.1099","volume":"63","author":[{"given":"Phoebus","family":"Tsaousoglou","sequence":"first","affiliation":[{"name":"School of Dental Medicine, Department of Periodontology, University of Bern, Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandor","family":"Nietzsche","sequence":"additional","affiliation":[{"name":"Department of Electron Microscopy, University Hospital of Jena, Jena, 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