{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T18:55:42Z","timestamp":1785869742846,"version":"3.56.0"},"reference-count":16,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,9,1]]},"abstract":"<jats:p>A total of 15 adult ewes from one flock known to be seroreactive for leptospirosis was studied. Urine and vaginal fluid were collected from each animal to test for the presence of leptospires using bacterial culture and conventional PCR methods. One pure culture of<jats:italic>Leptospira<\/jats:italic>sp. was obtained from the vaginal fluid sample of a non-pregnant ewe. The isolate was characterized by DNA sequencing of the<jats:italic>rrs<\/jats:italic>and<jats:italic>secY<\/jats:italic>genes, variable-number of tandem-repeats (VNTR) analysis and serogrouping, and the isolate was typed as<jats:italic>Leptospira<\/jats:italic><jats:italic>interrogans<\/jats:italic>serogroup Sejroe serovar Hardjo type Hardjoprajitno. This report indicates the presence of viable<jats:italic>Leptospira<\/jats:italic>in the vaginal fluid of a ewe, suggesting the potential for venereal transmission of leptospires in sheep.<\/jats:p>","DOI":"10.1099\/jmm.0.065466-0","type":"journal-article","created":{"date-parts":[[2014,6,17]],"date-time":"2014-06-17T05:50:55Z","timestamp":1402984255000},"page":"1234-1236","source":"Crossref","is-referenced-by-count":38,"title":["Isolation of Leptospira interrogans Hardjoprajitno from vaginal fluid of a clinically healthy ewe suggests potential for venereal transmission"],"prefix":"10.1099","volume":"63","author":[{"given":"A.","family":"Director","sequence":"first","affiliation":[{"name":"Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niter\u00f3i, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Penna","sequence":"additional","affiliation":[{"name":"Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niter\u00f3i, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Hamond","sequence":"additional","affiliation":[{"name":"Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niter\u00f3i, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. P.","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niter\u00f3i, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Martins","sequence":"additional","affiliation":[{"name":"Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niter\u00f3i, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Recombinant Technology Laboratory, Bio-Manguinhos, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Av. Brasil, 4365 \u2013 Manguinhos, Rio de Janeiro, RJ 21040-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Lilenbaum","sequence":"additional","affiliation":[{"name":"Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology, Fluminense Federal University, Rua Professor Ernani Pires de Melo 101, 24210-130 Niter\u00f3i, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","first-page":"175","article-title":"Leptospira interrogans serovar Hardjo in the kidneys and genital tracts of naturally infected sheep","volume":"19","author":"Cerri","year":"1996","journal-title":"New Microbiol"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1863-2378.2012.01470.x"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(96)90012-0"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2009.36865"},{"key":"r5","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.2460\/ajvr.1986.47.08.1694","article-title":"Isolation of leptospires from the genital tracts of Iowa cows","volume":"47","author":"Ellis","year":"1986","journal-title":"Am J Vet Res"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1136\/vr.118.1.11"},{"key":"r7","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.2460\/ajvr.1994.55.09.1232","article-title":"Sheep as maintenance host for Leptospira interrogans serovar Hardjo subtype Hardjobovis","volume":"55","author":"Gerritsen","year":"1994","journal-title":"Am J Vet Res"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1136\/vr.e5022"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-013-9582-x"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03474.x"},{"key":"r11","first-page":"164","article-title":"Leptospirosis in sheep in Western Canada","volume":"26","author":"Kingscote","year":"1985","journal-title":"Can Vet J"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.10.027"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822005000100009"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.2.539-545.2005"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-011-9964-4"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1136\/vr.100490"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.065466-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T10:42:54Z","timestamp":1746268974000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.065466-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,1]]},"references-count":16,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2014,9,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.065466-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,1]]}}}