{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,27]],"date-time":"2024-01-27T17:57:36Z","timestamp":1706378256173},"reference-count":27,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:p>\n                  <jats:italic>Pseudomonas aeruginosa<\/jats:italic> is a common cause of nosocomial infections and is associated with high rates of mortality. In order to facilitate rapid and sensitive identification of the most prevalent serotypes of <jats:italic>P. aeruginosa<\/jats:italic>, we have developed a 4-valent real-time PCR-based assay using oligonucleotides specific for open-reading frames constituting the O-antigen-specific lipopolysaccharide loci of <jats:italic>P. aeruginosa<\/jats:italic>. The assay simultaneously detects and differentiates between each of the four serotypes IATS-O1, -O6, -O11 and serogroup 2 (IATS-O2, -O5, and -O16) with high sensitivity and specificity in a single-tube reaction. No cross-reactivity was observed with other serotypes of <jats:italic>P. aeruginosa<\/jats:italic> or other microbial species, and reproducibility was demonstrated regardless of template, i.e. purified DNA, bacterial culture and clinical specimens (broncho-alveolar lavage). The limit of detection of the assay was approximately 100 copies per reaction for IATS-O1, -O2 and -O11, and 50 copies per reaction for IATS-O6. Comparison of the assay specificity with a commercially available slide agglutination kit showed consistent results; however, the number of non-typable isolates was reduced by 15\u200a% using the genotyping assay. Use of the 4-valent genotyping assay in the context of a clinical trial resulted in identification of pneumonia patients positive for the IATS-O11 serotype, and hence eligible for therapy with panobacumab (an investigational monoclonal antibody against the O-polysaccharide of serotype IATS-O11).<\/jats:p>","DOI":"10.1099\/jmm.0.066043-0","type":"journal-article","created":{"date-parts":[[2014,1,16]],"date-time":"2014-01-16T03:44:29Z","timestamp":1389843869000},"page":"508-517","source":"Crossref","is-referenced-by-count":3,"title":["Development of a 4-valent genotyping assay for direct identification of the most frequent Pseudomonas aeruginosa serotypes from respiratory specimens of pneumonia patients"],"prefix":"10.1099","volume":"63","author":[{"given":"Holger","family":"Koch","sequence":"first","affiliation":[{"name":"Kenta Biotech AG, Wagistrasse 25, CH-8952 Schlieren, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Emrich","sequence":"additional","affiliation":[{"name":"Kenta Biotech AG, Wagistrasse 25, CH-8952 Schlieren, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Jampen","sequence":"additional","affiliation":[{"name":"Kenta Biotech AG, Wagistrasse 25, CH-8952 Schlieren, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianne","family":"Wyss","sequence":"additional","affiliation":[{"name":"Kenta Biotech AG, Wagistrasse 25, CH-8952 Schlieren, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verena","family":"Gafner","sequence":"additional","affiliation":[{"name":"Kenta Biotech AG, Wagistrasse 25, CH-8952 Schlieren, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hedvika","family":"Lazar","sequence":"additional","affiliation":[{"name":"Kenta Biotech AG, Wagistrasse 25, CH-8952 Schlieren, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P.","family":"Rudolf","sequence":"additional","affiliation":[{"name":"Kenta Biotech AG, Wagistrasse 25, CH-8952 Schlieren, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200405-644ST"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1086\/377000"},{"key":"r3","first-page":"797","article-title":"The invasive (quantitative) diagnosis of ventilator-associated pneumonia","volume":"50","author":"Chastre","year":"2005","journal-title":"Respir Care"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg500"},{"key":"r5","article-title":"Antimicrobial resistance surveillance in Europe 2009. 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