{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:20:26Z","timestamp":1790421626303,"version":"4.1.0"},"reference-count":33,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:p>We determined the frequency of isolation of staphylococcal small-colony variants (SCVs) from 31 culture-positive patients undergoing revision of total hip prosthesis for aseptic loosening or presumed prosthetic-joint infection (PJI). We analysed auxotrophy of cultured SCVs, their antimicrobial susceptibility profiles and their biofilm-forming capacity. Eight SCV strains were cultivated from six (19\u200a%) patients. All SCVs were coagulase-negative staphylococci (CNS) with <jats:italic>Staphylococcus epidermidis<\/jats:italic> as the predominant species; there was also one <jats:italic>Staphylococcus warneri<\/jats:italic> SCV. The SCVs were auxotrophic for haemin, with one strain additionally auxotrophic for menadione. We noted the presence of two phenotypically (differences concerning antimicrobial susceptibility) and genetically distinct SCV strains in one patient, as well as the growth of two genetically related SCVs that differed in terms of their morphology and the type of auxotrophy in another. Seven out of eight SCVs were resistant to meticillin and gentamicin. In addition, antibiotic sensitivity testing revealed three multidrug-resistant SCV\u2013normal-morphology isolate pairs. One <jats:italic>S. epidermidis<\/jats:italic> SCV harboured <jats:italic>icaADBC<\/jats:italic> genes and was found to be a proficient biofilm producer. This paper highlights the involvement of CNS SCVs in the aetiology of PJIs, including what is believed to be the first report of a <jats:italic>S. warneri<\/jats:italic> SCV. These subpopulations must be actively sought in the routine diagnosis of implant-associated infections. Moreover, in view of the phenotypic and genetic diversity of some SCV pairs, particular attention should be paid to the investigation of all types of observed colony morphologies, and isolates should be subjected to antimicrobial susceptibility testing.<\/jats:p>","DOI":"10.1099\/jmm.0.066068-0","type":"journal-article","created":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T03:03:50Z","timestamp":1385003030000},"page":"176-185","source":"Crossref","is-referenced-by-count":44,"title":["Characterization of Staphylococcus epidermidis and Staphyloccocus warneri small-colony variants associated with prosthetic-joint infections"],"prefix":"10.1099","volume":"63","author":[{"given":"Agnieszka","family":"Bogut","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Medical University of Lublin, Chod\u017aki 1 Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna","family":"Nied\u017awiadek","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Medical University of Lublin, Chod\u017aki 1 Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Kozio\u0142-Montewka","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Medical University of Lublin, Chod\u017aki 1 Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dagmara","family":"Strzelec-Nowak","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Medical University of Lublin, Chod\u017aki 1 Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Blacha","sequence":"additional","affiliation":[{"name":"Orthopaedics and Traumatology Ward, Clinical Hospital No. 4, Jaczewskiego 8 Street, 20-954 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Mazurkiewicz","sequence":"additional","affiliation":[{"name":"Orthopaedics and Traumatology Ward, Clinical Hospital No. 4, Jaczewskiego 8 Street, 20-954 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Marczy\u0144ski","sequence":"additional","affiliation":[{"name":"Orthopaedics Ward, Public Clinical Hospital of Prof. Adam Gruca, Konarskiego 13 Street, 05-400 Otwock, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Plewik","sequence":"additional","affiliation":[{"name":"Research Centre for Innovation, Pope John Paul II State School of Higher Education in Bia\u0142a Podlaska, Sidorska 105 Street, 21-500 Bia\u0142a Podlaska, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-003-1029-9"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00160-07"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01395-07"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.02.382-388.2002"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.10.56"},{"key":"r6","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1128\/JCM.37.5.1306-1312.1999","article-title":"Phenotypic and genomic variation among Staphylococcus epidermidis strains infecting joint prostheses","volume":"37","author":"Galdbart","year":"1999","journal-title":"J Clin Microbiol"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt072"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1086\/515238"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.06.029"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02316-08"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1097\/01.blo.0000175715.68624.3d"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2010.10.025"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2011.09.026"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.1.95"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1384"},{"key":"r16","doi-asserted-by":"crossref","first-page":"2530","DOI":"10.1128\/JCM.36.9.2530-2534.1998","article-title":"Chronic prosthetic hip infection caused by a small-colony variant of Escherichia coli.","volume":"36","author":"Roggenkamp","year":"1998","journal-title":"J Clin Microbiol"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.11.046"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2009.10.005"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.3201\/eid1108.041177"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2008.11.004"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1086\/507633"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01562-09"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir402"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.009720-0"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.017046-0"},{"key":"r26","first-page":"243","article-title":"Prosthetic joint infections: update in diagnosis and treatment","volume":"135","author":"Trampuz","year":"2005","journal-title":"Swiss Med Wkly"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa061588"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1086\/656047"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2007.10.026"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1086\/516962"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1086\/520462"},{"key":"r32","doi-asserted-by":"publisher","DOI":"10.1086\/320519"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01353.x"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.066068-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T08:13:21Z","timestamp":1601972001000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.066068-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,1]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,2,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.066068-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,1]]}}}