{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:45:23Z","timestamp":1773283523695,"version":"3.50.1"},"reference-count":29,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:p>Killing rates (<jats:italic>K<\/jats:italic>) of 1\u201332 \u00b5g ml<jats:sup>\u22121<\/jats:sup> caspofungin were determined in RPMI-1640 and in 50\u200a% serum using time\u2013kill methodology against three <jats:italic>Candida krusei<\/jats:italic> (MICs of all three isolates 0.25 \u00b5g ml<jats:sup>\u22121<\/jats:sup> in RPMI-1640 and 2 \u00b5g ml<jats:sup>\u22121<\/jats:sup> in serum) and three <jats:italic>Candida inconspicua<\/jats:italic> clinical isolates (MIC ranges 0.06\u20130.12 \u00b5g ml<jats:sup>\u22121<\/jats:sup> in RPMI-1640 and 0.25\u20130.5 \u00b5g ml<jats:sup>\u22121<\/jats:sup> in serum), against <jats:italic>C. krusei<\/jats:italic> ATCC 6258 and against one <jats:italic>C. krusei<\/jats:italic> isolate that was resistant to echinocandins (MIC 8 \u00b5g ml<jats:sup>\u22121<\/jats:sup> in RPMI-1640 and 32 \u00b5g ml<jats:sup>\u22121<\/jats:sup> in serum). In RPMI-1640, the highest mean <jats:italic>K<\/jats:italic> values were observed at 4 (\u22121.05 h<jats:sup>\u22121<\/jats:sup>) and 16 (\u22120.27 h<jats:sup>\u22121<\/jats:sup>) \u03bcg ml<jats:sup>\u22121<\/jats:sup> caspofungin for <jats:italic>C. krusei<\/jats:italic> and <jats:italic>C. inconspicua<\/jats:italic> clinical isolates, respectively. In 50\u200a% serum, mean <jats:italic>K<\/jats:italic> value ranges at 1\u201332 and 4\u201332 \u00b5g ml<jats:sup>\u22121<\/jats:sup> concentrations for <jats:italic>C. inconspicua<\/jats:italic> and <jats:italic>C. krusei<\/jats:italic> were \u22121.12 to \u22121.44 and \u22120.42 to \u22120.57 h<jats:sup>\u22121<\/jats:sup>, respectively. While <jats:italic>K<\/jats:italic> values against <jats:italic>C. krusei<\/jats:italic> in RPMI-1640 and 50\u200a% serum were comparable, serum significantly increased the killing rate against <jats:italic>C. inconspicua<\/jats:italic> (<jats:italic>P<\/jats:italic>&lt;0.0003 for all tested concentrations). In a neutropenic murine model, daily caspofungin at 1, 2, 3, 5 and 15 mg kg<jats:sup>\u22121<\/jats:sup> significantly decreased the fungal tissue burden of <jats:italic>C. inconspicua<\/jats:italic> in the kidneys (<jats:italic>P<\/jats:italic>&lt;0.05\u20130.001). Against <jats:italic>C. krusei<\/jats:italic>, doses of 3, 5 and 15 mg kg<jats:sup>\u22121<\/jats:sup> caspofungin were effective (<jats:italic>P<\/jats:italic>&lt;0.05\u20130.01). All effective doses were comparably efficacious for both species. Only the highest 15 mg kg<jats:sup>\u22121<\/jats:sup> caspofungin dose was effective even against the echinocandin-resistant <jats:italic>C. krusei<\/jats:italic> isolate. In 50\u200a% serum, killing was concentration independent at effective concentrations (\u22654 and \u22651 \u00b5g ml<jats:sup>\u22121<\/jats:sup> for <jats:italic>C. krusei<\/jats:italic> and <jats:italic>C. inconspicua<\/jats:italic>, respectively), suggesting that the efficacy of dose escalation is questionable. These <jats:italic>in vitro<\/jats:italic> results were also supported by the murine model.<\/jats:p>","DOI":"10.1099\/jmm.0.066381-0","type":"journal-article","created":{"date-parts":[[2013,11,2]],"date-time":"2013-11-02T03:14:01Z","timestamp":1383362041000},"page":"186-194","source":"Crossref","is-referenced-by-count":15,"title":["Killing rates exerted by caspofungin in 50\u200a% serum and its correlation with in vivo efficacy in a neutropenic murine model against Candida krusei and Candida inconspicua"],"prefix":"10.1099","volume":"63","author":[{"given":"Ren\u00e1t\u00f3","family":"Kov\u00e1cs","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolf","family":"Gesztelyi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacodynamics, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00e9ka","family":"Ber\u00e9nyi","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianna","family":"Dom\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e1bor","family":"Kardos","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e9la","family":"Juh\u00e1sz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacodynamics, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1szl\u00f3","family":"Majoros","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01584-09"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1086\/598933"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00160-09"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01538-09"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.2165\/11585270-000000000-00000"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.12.3302-3305.2000"},{"key":"r7","article-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; 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