{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T01:56:50Z","timestamp":1789955810790,"version":"4.0.1"},"reference-count":43,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,1]]},"abstract":"<jats:p>A growing number of \u03b2-lactamases have been reported in <jats:italic>Pseudomonas aeruginosa<\/jats:italic> clinical isolates. The aim of this study was to investigate the diversity of \u03b2-lactamases in the collection of 51 ceftazidime-resistant <jats:italic>P. aeruginosa<\/jats:italic> clinical isolates in four hospitals of southern China. Among these isolates, variable degrees of resistance to other \u03b2-lactam and non-\u03b2-lactam agents were observed. Pulsed-field gel electrophoresis (PFGE) revealed a high degree of clonality with five main genotypes. Of the 51 isolates tested, 35 (68.6\u200a%) were identified as extended-spectrum \u03b2-lactamase (ESBL) producers, with 35 producing PER-1, 1 CTX-M-3, 7 CTX-M-15 and 1 CTX-M-14. Most (82.9\u200a%, 29\/35) PER-1-producing isolates were collected from two hospitals between January and April in 2008 and belonged to the same PFGE pattern (pattern B) with similar antibiogram and \u03b2-lactamase profiles, which suggested an outbreak of this clone at the time. The prevalence of CTX-M-type ESBL (17.6\u200a%, 9\/51) was unexpectedly high. One isolate was identified as producing VIM-2. Furthermore, we also reported an occurrence of a novel OXA-10 variant, OXA-246, in 14 <jats:italic>P. aeruginosa<\/jats:italic> isolates. In addition, AmpC overproduction was found to be the \u03b2-lactamase-mediated mechanism responsible for ceftazidime resistance in 6 isolates (11.8\u200a%). Our results revealed an overall diversity of \u03b2-lactamases and outbreak of a PER-1-producing clone among ceftazidime-resistant <jats:italic>P. aeruginosa<\/jats:italic> in southern China.<\/jats:p>","DOI":"10.1099\/jmm.0.069427-0","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T05:16:14Z","timestamp":1389158174000},"page":"386-392","source":"Crossref","is-referenced-by-count":20,"title":["Outbreak of PER-1 and diversity of \u03b2-lactamases among ceftazidime-resistant Pseudomonas aeruginosa clinical isolates"],"prefix":"10.1099","volume":"63","author":[{"given":"Yun","family":"Qing","sequence":"first","affiliation":[{"name":"Department of Immunology, Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China"},{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, PR China"},{"name":"Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou 510080, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Yuan","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Li","family":"Fang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou 510080, PR China"},{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, PR China"},{"name":"Department of Immunology, Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Min","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou 510080, PR China"},{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, PR China"},{"name":"Department of Immunology, Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Fei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou 510080, PR China"},{"name":"Department of Immunology, Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Bao","family":"Tian","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou 510080, PR China"},{"name":"Department of Immunology, Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou 510080, PR China"},{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, PR China"},{"name":"Department of Immunology, Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1586\/eri.10.119"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1179\/1120009X12Z.00000000015"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46704-0"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf069"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4030-4036.2002"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm319"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl055"},{"key":"r8","article-title":"Performance Standards for Antimicrobial Susceptibility Testing; 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