{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:02:34Z","timestamp":1791295354293,"version":"4.1.0"},"reference-count":53,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,7,1]]},"abstract":"<jats:p>Phenotypic and genetic characteristics of<jats:italic>Shigella<\/jats:italic>spp. isolated from diarrhoeal and asymptomatic children aged up to 5 years were analysed in this study. In total, 91 and 17 isolates were identified from diarrhoeal (case) and asymptomatic (control) children, respectively. All the isolates were tested for antimicrobial resistance, the presence of integrons, plasmid-mediated quinolone resistance (PMQR), virulence-associated genes and<jats:italic>Shigella<\/jats:italic>pathogenicity island (SH-PAI). The majority of the<jats:italic>Shigella<\/jats:italic>spp. from cases (68.1\u200a%) and controls (82.3\u200a%) were found to be resistant to fluoroquinolones. Integron carriage was detected more in cases (76.9\u200a%) than in controls (35.5\u200a%). Atypical class 1 integron was detected exclusively in<jats:italic>Shigella<\/jats:italic><jats:italic>flexneri<\/jats:italic>from cases but not from the controls. PMQR genes such as<jats:italic>aac(6\u2032)-Ib-cr<\/jats:italic>and<jats:italic>qnrS1<\/jats:italic>were detected in 82.4\u200a and 14.3\u200a% of the isolates from cases and in 53 and 17.6\u200a% in controls, respectively.<jats:italic>Shigella<\/jats:italic>isolates from cases as well as from controls were positive for the invasive plasmid antigen H-encoding gene<jats:italic>ipaH<\/jats:italic>. The other virulence genes such as<jats:italic>virF<\/jats:italic>,<jats:italic>sat<\/jats:italic>,<jats:italic>setA<\/jats:italic>,<jats:italic>setB<\/jats:italic>,<jats:italic>sen<\/jats:italic>and<jats:italic>ial<\/jats:italic>were detected in<jats:italic>Shigella<\/jats:italic>isolates in 80.2, 49.4, 27.4, 27.4, 80.2 and 79.1\u200a% of cases and in 64.7, 52.9, 17.6, 17.6, 64.7 and 64.7\u200a% of controls, respectively. The entire SH-PAI was detected in<jats:italic>S. flexneri<\/jats:italic>serotype 2a from cases and controls. In an isolate from a control child, the SH-PAI was truncated. Integrons, PMQR and virulence-encoding genes were detected more frequently in cases than in controls. In diarrhoea endemic areas, asymptomatic carriers may play a crucial role in the transmission of multidrug-resistant<jats:italic>Shigella<\/jats:italic>spp. with all the putative virulence genes.<\/jats:p>","DOI":"10.1099\/jmm.0.070912-0","type":"journal-article","created":{"date-parts":[[2014,5,2]],"date-time":"2014-05-02T03:09:39Z","timestamp":1399000179000},"page":"903-910","source":"Crossref","is-referenced-by-count":25,"title":["Genetic characterization of Shigella spp. isolated from diarrhoeal and asymptomatic children"],"prefix":"10.1099","volume":"63","author":[{"given":"Santanu","family":"Ghosh","sequence":"first","affiliation":[{"name":"Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gururaja P.","family":"Pazhani","sequence":"additional","affiliation":[{"name":"Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, 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