{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T21:53:18Z","timestamp":1788990798268,"version":"build-2803163510"},"reference-count":33,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,10,1]]},"abstract":"<jats:p>S<jats:italic>taphylococcus epidermidis<\/jats:italic> is the most commonly isolated aetiological agent of nosocomial infections, mainly due to its ability to establish biofilms on indwelling medical devices. Detachment of bacteria from <jats:italic>S. epidermidis<\/jats:italic> biofilms and subsequent growth in the planktonic form is a hallmark of the pathogenesis of these infections leading to dissemination. Here we showed that <jats:italic>S. epidermidis<\/jats:italic> cells collected from biofilms cultured in conditions that promote cell viability present marked changes in their physiological status upon initiating a planktonic mode of growth. When compared to cells growing in biofilms, they displayed an increased SYBR green I staining intensity, increased transcription of the <jats:italic>rpiA<\/jats:italic> gene, decreased transcription of the <jats:italic>icaA<\/jats:italic> gene, as well as higher susceptibility to vancomycin and penicillin. When bacteria collected from biofilms with high proportions of dormant cells were subsequently cultured in the planktonic mode, a large proportion of cells maintained a low SYBR green I staining intensity and increased resistance to vancomycin and penicillin, a profile typical of dormant cells. This phenotype further associated with a decreased ability of these biofilm-derived cells to induce the production of pro-inflammatory cytokines by bone marrow-derived dendritic cells <jats:italic>in vitro<\/jats:italic>. These results demonstrated that cells detached from the biofilm maintain a dormant cell-like phenotype, having a low pro-inflammatory effect and decreased susceptibility to antibiotics, suggesting these cells may contribute to the recalcitrant nature of biofilm infections.<\/jats:p>","DOI":"10.1099\/jmm.0.073163-0","type":"journal-article","created":{"date-parts":[[2014,7,23]],"date-time":"2014-07-23T04:50:35Z","timestamp":1406091035000},"page":"1274-1283","source":"Crossref","is-referenced-by-count":32,"title":["Dormant bacteria within Staphylococcus epidermidis biofilms have low inflammatory properties and maintain tolerance to vancomycin and penicillin after entering planktonic growth"],"prefix":"10.1099","volume":"63","author":[{"given":"Filipe","family":"Cerca","sequence":"first","affiliation":[{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular, Rua do Campo Alegre 83, Porto, Portugal"},{"name":"ICBAS-UP \u2013 Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar 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