{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T09:30:08Z","timestamp":1789810208477,"version":"4.0.1"},"reference-count":53,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,10,1]]},"abstract":"<jats:p>Ten carbapenem-resistant<jats:italic>Enterobacteriaceae<\/jats:italic>(eight<jats:italic>Klebsiella pneumoniae<\/jats:italic>isolates and two<jats:italic>Enterobacter cloacae<\/jats:italic>) isolates from Yemen were investigated using<jats:italic>in vitro<\/jats:italic>antimicrobial susceptibility testing, phenotypic carbapenemase detection, multilocus sequence typing (MLST) and replicon typing. Carbapenemase, extended-spectrum \u03b2-lactamase (ESBL) and plasmid-mediated quinolone resistance determinant genes were identified using PCR and sequencing. All of the 10 carbapenem-resistant<jats:italic>Enterobacteriaceae<\/jats:italic>were resistant to \u03b2-lactams, tobramycin, ciprofloxacin and cotrimoxazole. Imipenem, doripenem and meropenem MICs ranged from 2 to &gt;32 mg l<jats:sup>\u22121<\/jats:sup>and ertapenem MICs ranged from 6 to &gt;32 mg l<jats:sup>\u22121<\/jats:sup>. All of the<jats:italic>K. pneumoniae<\/jats:italic>isolates showed ESBL activity in phenotypic tests. Genes encoding<jats:italic>bla<\/jats:italic><jats:sub>NDM<\/jats:sub>were detected in all strains. All<jats:italic>K. pneumoniae<\/jats:italic>strains produced CTX-M-15 ESBL and SHV \u03b2-lactamases. TEM-1 \u03b2-lactamase was detected in seven isolates. Nine isolates were<jats:italic>qnr<\/jats:italic>positive including QnrB1, QnrA1 and QnrS1, and six isolates produced AAC-6\u2032-Ib-cr. MLST identified five different sequence types (STs): ST1399, ST147, ST29, ST405 and ST340. Replicon typing showed the presence of IncFII1K plasmids in four transformants. 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