{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T13:08:00Z","timestamp":1648732080807},"reference-count":47,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,9,1]]},"abstract":"<jats:p>Leptospirosis, a worldwide zoonotic infection, is an important human and veterinary health problem. We have previously identified a leptospiral multipurpose adhesin, Lsa66, capable of binding extracellular matrix (ECM) components and plasminogen (PLG). In this work, we report the cloning, expression, purification and characterization of three fragments derived from the full-length Lsa66: N-terminal, intermediate and C-terminal regions. We employed <jats:italic>Escherichia coli<\/jats:italic> BL21-SI as expression cells. The recombinant fragments tagged with N-terminal His<jats:sub>6<\/jats:sub> were purified by metal-charged chromatography to major protein bands that were recognized by anti-His-tag mAbs. The recombinant fragments were evaluated for their capacity to attach to ECM components and to PLG. The intermediate region bound to laminin, plasma fibronectin and PLG. Laminin also bound to the C-terminal region. Antibodies in leptospirosis-positive serum samples recognized Lsa66, being the immune epitopes located at the N-terminal and intermediate fragments. The data confirm that Lsa66 is expressed during infection and that this protein might have a role in bacterial infection.<\/jats:p>","DOI":"10.1099\/jmm.0.078378-0","type":"journal-article","created":{"date-parts":[[2014,6,16]],"date-time":"2014-06-16T13:48:59Z","timestamp":1402926539000},"page":"1119-1130","source":"Crossref","is-referenced-by-count":0,"title":["Intermediate and C-terminal regions of leptospiral adhesin Lsa66 are responsible for binding with plasminogen and extracellular matrix components"],"prefix":"10.1099","volume":"63","author":[{"given":"Rosane","family":"Oliveira","sequence":"first","affiliation":[{"name":"Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, USP, Avenida Prof. Lineu Prestes, 1730, 05508-900 S\u00e3o Paulo, SP, Brazil"},{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renan F.","family":"Domingos","sequence":"additional","affiliation":[{"name":"Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, USP, Avenida Prof. Lineu Prestes, 1730, 05508-900 S\u00e3o Paulo, SP, Brazil"},{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zenaide M.","family":"de Morais","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr Orlando Marques de Paiva, 87, 05508-270 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvio A.","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr Orlando Marques de Paiva, 87, 05508-270 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivy J.","family":"Alves","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz, Laborat\u00f3rio Regional de Santos, N\u00facleo de Ci\u00eancias Biom\u00e9dicas, Rua Silva Jardim, 90, 11015-020, Santos, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliete C.","family":"Romero","sequence":"additional","affiliation":[{"name":"Centro de Bacteriologia, Instituto Adolfo Lutz, Av. 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