{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T18:18:45Z","timestamp":1788113925588,"version":"build-2803163510"},"reference-count":26,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,11,1]]},"abstract":"<jats:p>Fungaemia caused by <jats:italic>Malassezia<\/jats:italic> spp. in hospitalized patients requires prompt and appropriate therapy, but standard methods for the definition of the <jats:italic>in vitro<\/jats:italic> antifungal susceptibility have not been established yet. In this study, the <jats:italic>in vitro<\/jats:italic> susceptibility of <jats:italic>Malassezia furfur<\/jats:italic> from bloodstream infections (BSIs) to amphotericin B (AMB), fluconazole (FLC), itraconazole (ITC), posaconazole (POS) and voriconazole (VRC) was assessed using the broth microdilution (BMD) method of the Clinical and Laboratory Standards Institute (CLSI) with different media such as modified Sabouraud dextrose broth (SDB), RPMI and Christensen\u2019s urea broth (CUB). Optimal broth media that allow sufficient growth of <jats:italic>M. furfur<\/jats:italic>, and produce reliable and reproducible MICs using the CLSI BMD protocol were assessed. Thirty-six <jats:italic>M. furfur<\/jats:italic> isolates collected from BSIs of patients before and during AMB therapy, and receiving FLC prophylaxis, were tested. A good growth of <jats:italic>M. furfur<\/jats:italic> was observed in RPMI, CUB and SDB at 32 \u00b0C for 48 and 72 h. No statistically significant differences were detected between the MIC values registered after 48 and 72 h incubation. ITC, POS and VRC displayed lower MICs than FLC and AMB. These last two antifungal drugs showed higher and lower MICs, respectively, when the isolates were tested in SDB. SDB is the only medium in which it is possible to detect isolates with high FLC MICs in patients receiving FLC prophylaxis. A large number of isolates showed high AMB MIC values regardless of the media used. In conclusion, SDB might be suitable to determine triazole susceptibility. However, the media, the drug formulation or the breakpoints herein applied might not be useful for assessing the AMB susceptibility of <jats:italic>M. furfur<\/jats:italic> from BSIs.<\/jats:p>","DOI":"10.1099\/jmm.0.078709-0","type":"journal-article","created":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T03:18:09Z","timestamp":1409282289000},"page":"1467-1473","source":"Crossref","is-referenced-by-count":44,"title":["In vitro antifungal susceptibility of Malassezia furfur from bloodstream infections"],"prefix":"10.1099","volume":"63","author":[{"given":"Roberta","family":"Iatta","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 degli Studi di Bari Aldo Moro, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana A.","family":"Figueredo","sequence":"additional","affiliation":[{"name":"Department of Immunology, Aggeu Magalh\u00e3es Research Centre, FIOCRUZ, Recife-PE, Brazil"},{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 degli Studi di Bari Aldo Moro, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Montagna","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Biomediche e Oncologia Umana, Universit\u00e0 degli Studi di Bari Aldo Moro, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Otranto","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 degli Studi di Bari Aldo Moro, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Cafarchia","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria, Universit\u00e0 degli Studi di Bari Aldo Moro, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12360"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701191373"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt232"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2010.12.003"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.04.034"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.09.008"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12076"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1080\/080352501300067712"},{"key":"r9","article-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; 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