{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:49:29Z","timestamp":1787010569202,"version":"build-2736575974"},"reference-count":35,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:p>\n                    Antibiotic-resistant bacteria have emerged from the widespread use of antibiotics worldwide and have prompted the search for new sources of antimicrobial substances.\n                    <jats:italic>Pinus<\/jats:italic>\n                    spp. contain several bioactive compounds consisting mainly of terpenes, terpenoids and some other aromatic and aliphatic constituents. These compounds exert important biological effects, and pine oils have found wide application in the industry. In the present study, we have evaluated the potential activity of the resin-oil of\n                    <jats:italic>Pinus elliottii<\/jats:italic>\n                    and its major compound dehydroabietic acid (DA) against multiresistant bacteria by MIC, minimum bactericidal concentration and time-kill assays. The MIC of the resin-oil of\n                    <jats:italic>P. elliottii<\/jats:italic>\n                    varied between 25 and 100 \u00b5g ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    . As for DA, the MIC and minimum bactericidal concentration varied between 6.25 and 50 and between 6.25 and 100 \u00b5g ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively. The time-kill assay conducted with DA at 6.25 \u00b5g ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    evidenced bactericidal activity against\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    (American Type Culture Collection 14990) within 24 h. On the basis of these results, the resin-oil of\n                    <jats:italic>P. elliottii<\/jats:italic>\n                    and its major compound DA play an important part in the search for novel sources of agents that can act against multiresistant bacteria.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.081711-0","type":"journal-article","created":{"date-parts":[[2014,9,27]],"date-time":"2014-09-27T02:53:36Z","timestamp":1411786416000},"page":"1649-1653","source":"Crossref","is-referenced-by-count":47,"title":["Antibacterial activity of Pinus elliottii and its major compound, dehydroabietic acid, against multidrug-resistant strains"],"prefix":"10.1099","volume":"63","author":[{"given":"Lu\u00eds Fernando","family":"Leandro","sequence":"first","affiliation":[{"name":"Laboratory of Research in Applied Microbiology, University of Franca (UNIFRAN), Franca, 14404-600, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel Jorge Oliveira","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Laboratory of Research in Applied Microbiology, University of Franca (UNIFRAN), Franca, 14404-600, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro Donizeti Caetano","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Research in Applied Microbiology, University of Franca (UNIFRAN), Franca, 14404-600, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Gorete Mendes","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Research in Applied Microbiology, University of Franca (UNIFRAN), Franca, 14404-600, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Cassio Sola","family":"Veneziani","sequence":"additional","affiliation":[{"name":"Nucleus of Research in Sciences and Technology, University of Franca (UNIFRAN), 14404-600, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio Ricardo","family":"Ambrosio","sequence":"additional","affiliation":[{"name":"Nucleus of Research in Sciences and Technology, University of Franca (UNIFRAN), 14404-600, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Henrique Gomes","family":"Martins","sequence":"additional","affiliation":[{"name":"Laboratory of Research in Applied Microbiology, University of Franca (UNIFRAN), Franca, 14404-600, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(00)00335-4"},{"key":"r2","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1515\/znc-2008-5-603","article-title":"Antimicrobial activity of kaurane diterpenes against oral pathogens","volume":"63","author":"Ambrosio","year":"2008","journal-title":"Z Naturforsch C"},{"key":"r3","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1590\/S0100-40422010000900022","article-title":"Colloidal aspects of bacterial adhesion","volume":"33","author":"Ara\u00fajo","year":"2010","journal-title":"Quim Nova"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707607"},{"key":"r5","doi-asserted-by":"crossref","first-page":"4725","DOI":"10.1128\/JB.181.16.4725-4733.1999","article-title":"Structures of Gram-negative cell walls and their derived membrane vesicles","volume":"181","author":"Beveridge","year":"1999","journal-title":"J Bacteriol"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2004.03.022"},{"key":"r7","volume-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; 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