{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:40:54Z","timestamp":1791240054667,"version":"4.1.0"},"reference-count":20,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,7,1]]},"abstract":"<jats:p>Between January 2002 and July 2003, 173 bronchoalveolar lavage (BAL) specimens from 150 patients (19 HIV-infected and 131 non-HIV-infected patients) were evaluated for identification of <jats:italic>Pneumocystis jiroveci<\/jats:italic> (formerly known as <jats:italic>Pneumocystis carinii<\/jats:italic> f. sp. <jats:italic>hominis<\/jats:italic>) using staining techniques, conventional PCR (<jats:italic>mtLSUrRNA<\/jats:italic> gene) and real-time PCR (<jats:italic>MSG<\/jats:italic> gene). Test results were compared to <jats:italic>Pneumocystis<\/jats:italic> pneumonia (PCP) confirmed by typical clinical findings and response to treatment. Sensitivity and specificity of the techniques were 60 and 100 % for staining (where either one or both techniques were positive), 100 and 87.0 % for conventional PCR and 100 and 84.9 % for real-time PCR, respectively. The use of a concentration of 10<jats:sup>3<\/jats:sup> copies of DNA per capillary of BAL as a cut-off (determined by real-time PCR) increased specificity from 84.9 to 98.6 % without reducing the sensitivity of the technique. This technique is rapid (&lt;3 h) and therefore of major interest in differentiating between asymptomatic carriage and PCP. A BAL specimen with &lt;10<jats:sup>3<\/jats:sup> copies per capillary of <jats:italic>Pneumocystis<\/jats:italic>-specific DNA is more likely to indicate a chronic carrier state, but in such cases follow-up is required to ensure that the patient is not in the early stage of an active PCP.<\/jats:p>","DOI":"10.1099\/jmm.0.45528-0","type":"journal-article","created":{"date-parts":[[2004,6,7]],"date-time":"2004-06-07T18:15:55Z","timestamp":1086632155000},"page":"603-607","source":"Crossref","is-referenced-by-count":166,"title":["Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jiroveci pneumonia from bronchoalveolar lavage specimens"],"prefix":"10.1099","volume":"53","author":[{"given":"Pierre","family":"Flori","sequence":"first","affiliation":[{"name":"Laboratory of Parasitology and Mycology, H\u00f4pital Nord1 and Department of Infectious and Tropical Diseases, H\u00f4pital Bellevue2, University Hospital of Saint Etienne, 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