{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T01:17:38Z","timestamp":1773451058091,"version":"3.50.1"},"reference-count":30,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,12,1]]},"abstract":"<jats:p>\n                  <jats:italic>Bartonella henselae<\/jats:italic> is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. A recombinant clone expressing an immunoreactive antigen of <jats:italic>B. henselae<\/jats:italic> was isolated by screening a genomic DNA cosmid library by Western blotting with sera pooled from patients positive for <jats:italic>B. henselae<\/jats:italic> IgG antibodies by indirect immunofluorescence (IFA). The deduced amino acid sequence of the 43.7 kDa encoded protein was found to be 76.3 % identical to the dihydrolipoamide succinyltransferase enzyme (SucB) of <jats:italic>Brucella melitensis<\/jats:italic>. SucB has been shown to be an immunogenic protein during infections by <jats:italic>Brucella melitensis<\/jats:italic>, <jats:italic>Coxiella burnetii<\/jats:italic> and <jats:italic>Bartonella vinsonii<\/jats:italic>. The agreement between reactivity with a recombinant SucB fusion protein on immunoblot analysis and the results obtained by IFA was 55 % for IFA-positive sera and 88 % for IFA-negative sera. Cross-reactivity was observed with sera from patients with antibodies against <jats:italic>Brucella melitensis<\/jats:italic>, <jats:italic>Mycoplasma pneumoniae<\/jats:italic>, <jats:italic>Francisella tularensis<\/jats:italic>, <jats:italic>Coxiella burnetii<\/jats:italic> and <jats:italic>Rickettsia typhi<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/jmm.0.45616-0","type":"journal-article","created":{"date-parts":[[2004,11,19]],"date-time":"2004-11-19T19:48:14Z","timestamp":1100893694000},"page":"1221-1227","source":"Crossref","is-referenced-by-count":13,"title":["The Bartonella henselae sucB gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease"],"prefix":"10.1099","volume":"53","author":[{"given":"Christine M","family":"Litwin","sequence":"first","affiliation":[{"name":"Section of Clinical Immunology, Microbiology and Virology, Department of Pathology, University of Utah, 50 N. Medical Drive, Salt Lake City, UT 84132, USA 2Associated Regional and University Pathologists (ARUP), Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel M","family":"Johnson","sequence":"additional","affiliation":[{"name":"Section of Clinical Immunology, Microbiology and Virology, Department of Pathology, University of Utah, 50 N. Medical Drive, Salt Lake City, UT 84132, USA 2Associated Regional and University Pathologists (ARUP), Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas B","family":"Martins","sequence":"additional","affiliation":[{"name":"Section of Clinical Immunology, Microbiology and Virology, Department of Pathology, University of Utah, 50 N. Medical Drive, Salt Lake City, UT 84132, USA 2Associated Regional and University Pathologists (ARUP), Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"H1-8","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"H2-8","doi-asserted-by":"crossref","first-page":"2358","DOI":"10.1128\/JCM.33.9.2358-2365.1995","article-title":"Characterization of a 17-kilodalton antigen of Bartonella henselae reactive with sera from patients with cat scratch disease","volume":"33","author":"Anderson","year":"1995","journal-title":"J Clin Microbiol"},{"key":"H3-8","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0378-1119(96)00330-7","article-title":"Cloning, expression and sequence analysis of the Bartonella henselae gene encoding the HtrA stress-response protein","volume":"178","author":"Anderson","year":"1996","journal-title":"Gene"},{"key":"H4-8","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1128\/JCM.35.8.1931-1937.1997","article-title":"Pitfalls and fallacies of cat scratch disease serology: evaluation of Bartonella henselae -based indirect fluorescence assay and enzyme-linked immunoassay","volume":"35","author":"Bergmans","year":"1997","journal-title":"J Clin Microbiol"},{"key":"H5-8","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"H6-8","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1016\/S0140-6736(88)92146-0","article-title":"Epithelioid angiomatosis and cat scratch disease bacillus","volume":"i","author":"Cockerell","year":"1988","journal-title":"Lancet"},{"key":"H7-8","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1001\/archinte.1995.00430150164017","article-title":"Use of Bartonella antigens for serologic diagnosis of cat-scratch disease at a national referral center","volume":"155","author":"Dalton","year":"1995","journal-title":"Arch Intern Med"},{"key":"H8-8","doi-asserted-by":"crossref","first-page":"331","DOI":"10.7326\/0003-4819-118-5-199303010-00002","article-title":"Syndrome of Rochalimaea henselae adenitis suggesting cat scratch disease","volume":"118","author":"Dolan","year":"1993","journal-title":"Ann Intern Med"},{"key":"H9-8","volume-title":"Statistical Methods for Rates and Proportions","author":"Fleiss","year":"1981"},{"key":"H10-8","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1128\/CDLI.6.4.558-566.1999","article-title":"Identification of Bartonella -specific immunodominant antigens recognized by the feline humoral immune system","volume":"6","author":"Freeland","year":"1999","journal-title":"Clin Diagn Lab Immunol"},{"key":"H11-8","doi-asserted-by":"crossref","first-page":"4818","DOI":"10.1128\/IAI.71.8.4818-4822.2003","article-title":"Molecular characterization of the sucB gene encoding the immunogenic dihydrolipoamide succinyltransferase protein of Bartonella vinsonii subsp. berkhoffii and Bartonella quintana","volume":"71","author":"Gilmore","year":"2003","journal-title":"Infect Immun"},{"key":"H12-8","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/ng0393-266","article-title":"Identification of protein coding regions by database similarity search","volume":"3","author":"Gish","year":"1993","journal-title":"Nat Genet"},{"key":"H13-8","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.2105\/AJPH.83.12.1707","article-title":"Cat scratch disease in the United States","volume":"83","author":"Jackson","year":"1993","journal-title":"Am J Public Health"},{"key":"H14-8","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s00203-001-0364-4","article-title":"Biochemical and antigenic characterisation of Mycoplasma gallisepticum membrane proteins P52 and P67 (pMGA","volume":"177","author":"Jan","year":"2001","journal-title":"Arch Microbiol"},{"key":"H15-8","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1001\/jama.1994.03510310061039","article-title":"Rochalimaea henselae infection.A new zoonosis with the domestic cat as reservoir","volume":"271","author":"Koehler","year":"1994","journal-title":"JAMA"},{"key":"H16-8","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.1128\/JCM.34.9.2270-2274.1996","article-title":"Serological cross-reactions between Bartonella quintana , Bartonella henselae , and Coxiella burnetii","volume":"34","author":"La Scola","year":"1996","journal-title":"J Clin Microbiol"},{"key":"H17-8","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1128\/CDLI.7.4.617-624.2000","article-title":"Differentiation of Bartonella species by a microimmunofluorescence assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western immunoblotting","volume":"7","author":"Liang","year":"2000","journal-title":"Clin Diagn Lab Immunol"},{"key":"H18-8","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1093\/ajcp\/108.2.202","article-title":"Immunologic response to Bartonella henselae as determined by enzyme immunoassay and Western blot analysis","volume":"108","author":"Litwin","year":"1997","journal-title":"Am J Clin Pathol"},{"key":"H19-8","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1128\/JCM.32.5.1166-1171.1994","article-title":"Isolation and characterization by immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot, restriction fragment length polymorphism-PCR, 16S rRNA gene sequencing, and pulsed-field gel electrophoresis of Rochalimaea quintana from a patient with bacillary angiomatosis","volume":"32","author":"Maurin","year":"1994","journal-title":"J Clin Microbiol"},{"key":"H20-8","doi-asserted-by":"crossref","first-page":"2283","DOI":"10.1128\/JCM.35.9.2283-2287.1997","article-title":"Serological cross-reactions between Bartonella and Chlamydia species: implications for diagnosis","volume":"35","author":"Maurin","year":"1997","journal-title":"J Clin Microbiol"},{"key":"H21-8","first-page":"1004","article-title":"Comparison of in-house and commercial slides for detection by immunofluorescence of immunoglobulins G and M against Bartonella henselae and Bartonella quintana","volume":"9","author":"Maurin","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"key":"H22-8","doi-asserted-by":"crossref","first-page":"5915","DOI":"10.1128\/IAI.66.12.5915-5920.1998","article-title":"Characterization of human immunoglobulin (Ig) isotype and IgG subclass response to Bartonella henselae infection","volume":"66","author":"McGill","year":"1998","journal-title":"Infect Immun"},{"key":"H23-8","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1097\/00000478-199207000-00003","article-title":"Immunocytochemical identification of Rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia","volume":"16","author":"Reed","year":"1992","journal-title":"Am J Surg Pathol"},{"key":"H24-8","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1016\/0140-6736(92)92032-B","article-title":"Serological response to \u2018\u2018 Rochalimaea henselae \u2019\u2019 antigen in suspected cat-scratch disease","volume":"339","author":"Regnery","year":"1992","journal-title":"Lancet"},{"key":"H25-8","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"H26-8","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1128\/CDLI.5.4.486-490.1998","article-title":"Seroprevalence of antibodies to Bartonella henselae in patients with cat scratch disease and in healthy controls: evaluation and comparison of two commercial serological tests","volume":"5","author":"Sander","year":"1998","journal-title":"Clin Diagn Lab Immunol"},{"key":"H27-8","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1128\/JB.151.2.542-552.1982","article-title":"Molecular cloning of four tricarboxylic acid cycle genes of Escherichia coli","volume":"151","author":"Spencer","year":"1982","journal-title":"J Bacteriol"},{"key":"H28-8","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1128\/CDLI.7.5.734-738.2000","article-title":"Prevalence of Chlamydia pneumoniae and Mycoplasma pneumoniae immunoglobulin G and A antibodies in a healthy Finnish population as analyzed by quantitative enzyme immunoassays","volume":"7","author":"Tuuminen","year":"2000","journal-title":"Clin Diagn Lab Immunol"},{"key":"H29-8","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1056\/NEJM199307013290102","article-title":"Cat scratch disease in Connecticut.Epidemiology, risk factors, and evaluation of a new diagnostic test","volume":"329","author":"Zangwill","year":"1993","journal-title":"N Engl J Med"},{"key":"H30-8","doi-asserted-by":"crossref","first-page":"6537","DOI":"10.1128\/IAI.69.10.6537-6540.2001","article-title":"Cloning, nucleotide sequence, and expression of the Brucella melitensis sucB gene coding for an immunogenic dihydrolipoamide succinyltransferase homologous protein","volume":"69","author":"Zygmunt","year":"2001","journal-title":"Infect Immun"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.45616-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T03:36:56Z","timestamp":1600832216000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.45616-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12,1]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2004,12,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.45616-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,12,1]]}}}