{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:44:31Z","timestamp":1790196271830,"version":"4.1.0"},"reference-count":14,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,9,1]]},"DOI":"10.1099\/jmm.0.45630-0","type":"journal-article","created":{"date-parts":[[2004,8,17]],"date-time":"2004-08-17T00:45:28Z","timestamp":1092703528000},"page":"875-878","source":"Crossref","is-referenced-by-count":32,"title":["Validation of a PCR for diagnosis of typhoid fever and salmonellosis by amplification of the hilA gene in clinical samples from Colombian patients"],"prefix":"10.1099","volume":"53","author":[{"given":"M. M.","family":"S\u00e1nchez-Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda, Instituto Colombiano de Medicina Tropical, Cra 43a No. 52 Sur 99, Sabaneta, Colombia 2Facultad de Medicina Universidad de Antioquia Programa de Maestr\u00eda Ciencias B\u00e1sicas Biom\u00e9dicas, Colombia 3Instituto de Ciencias de la Salud CES, Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Cardona-Castro","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda, Instituto Colombiano de Medicina Tropical, Cra 43a No. 52 Sur 99, Sabaneta, Colombia 2Facultad de Medicina Universidad de Antioquia Programa de Maestr\u00eda Ciencias B\u00e1sicas Biom\u00e9dicas, Colombia 3Instituto de Ciencias de la Salud CES, Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"H1-7","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1590\/S0074-02762002000800016","article-title":"Detection of hilA gene sequences in serovars of Salmonella enterica subspecies Enterica","volume":"97","author":"Cardona-Castro","year":"2002","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"H2-7","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1128\/JCM.39.5.1871-1876.2001","article-title":"Detection of salmonellae in chicken feces by a combination of tetrathionate broth enrichment, capillary PCR and capillary gel electrophoresis","volume":"39","author":"Carli","year":"2001","journal-title":"J Clin Microbiol"},{"key":"H3-7","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1128\/JCM.34.10.2619-2622.1996","article-title":"Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and spvC , by an enrichment broth culture-multiplex PCR combination assay","volume":"34","author":"Chiu","year":"1996","journal-title":"J Clin Microbiol"},{"key":"H4-7","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1093\/infdis\/161.6.1252","article-title":"Detection of Shigella in feces using DNA amplification","volume":"161","author":"Frankel","year":"1990","journal-title":"J Infect Dis"},{"key":"H5-7","doi-asserted-by":"crossref","first-page":"5248","DOI":"10.1128\/AEM.66.12.5248-5252.2000","article-title":"PCR detection of Salmonella enterica serotype Montevideo in and on raw tomatoes using primers derived from hilA","volume":"66","author":"Guo","year":"2000","journal-title":"Appl Environ Microbiol"},{"key":"H6-7","doi-asserted-by":"crossref","first-page":"337","DOI":"10.5144\/0256-4947.1999.337","article-title":"Early detection of typhoid by polymerase chain reaction","volume":"7","author":"Haque","year":"1999","journal-title":"Ann Saudi Medicine"},{"key":"H7-7","doi-asserted-by":"crossref","first-page":"5835","DOI":"10.1128\/JCM.41.12.5835.2003","article-title":"Making internal amplification control mandatory for diagnostic PCR","volume":"41","author":"Hoorfar","year":"2003","journal-title":"J Clin Microbiol"},{"key":"H8-7","volume-title":"Clinical Microbiology Procedures Handbook","author":"Isenberg","year":"1992"},{"key":"H9-7","first-page":"87","article-title":"Studying the accuracy and usefulness of screening and diagnostic tests","volume-title":"In Epidemiology, Biostatistics, and Preventive Medicine","author":"Jekel","year":"1996"},{"key":"H10-7","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1046\/j.1365-2958.2000.01991.x","article-title":"The cis requirements for transcriptional activation by HilA, a virulence determinant encoded on SPI-1","volume":"37","author":"Lostroh","year":"2000","journal-title":"Mol Microbiol"},{"key":"H11-7","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0168-1605(03)00154-5","article-title":"Interlaboratory diagnostic accuracy of a Salmonella specific PCR-based method","volume":"89","author":"Malorny","year":"2003","journal-title":"Int J Food Microbiol"},{"key":"H12-7","first-page":"2344","article-title":"Salmonella species, including Salmonella typhi . In Mandell, Douglas and Bennett's Principles and Practice of Infectious Diseases","author":"Miller","year":"2000"},{"key":"H13-7","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1128\/JB.183.7.2348-2358.2001","article-title":"Salmonella host cell invasion emerged by acquisition of a mosaic of separate genetic elements, including Salmonella pathogenicity island1 (SPI1), SPI5, and sopE2","volume":"183","author":"Mirold","year":"2001","journal-title":"J Bacteriol"},{"key":"H14-7","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1099\/jmm.0.05188-0","article-title":"Simple and rapid detection of Salmonella strains by direct PCR amplification of the hilA gene","volume":"52","author":"Pathmanathan","year":"2003","journal-title":"J Med Microbiol"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.45630-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T13:52:21Z","timestamp":1584539541000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.45630-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,1]]},"references-count":14,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2004,9,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.45630-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9,1]]}}}