{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:52:47Z","timestamp":1791150767097,"version":"4.1.0"},"reference-count":47,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,11,1]]},"abstract":"<jats:p>\n                  <jats:italic>Burkholderia pseudomallei<\/jats:italic> is the causative agent of human and animal melioidosis. The role of quorum sensing (QS) in the <jats:italic>in vivo<\/jats:italic> pathogenicity of <jats:italic>B. pseudomallei<\/jats:italic> via inhalational exposure of BALB\/c mice and intraperitoneal challenge of Syrian hamsters has not been reported. This investigation demonstrates that <jats:italic>B. pseudomallei<\/jats:italic> encodes a minimum of three <jats:italic>luxI<\/jats:italic> and five <jats:italic>luxR<\/jats:italic> homologues that are involved in animal pathogenicity. Mass spectrometry analysis of culture supernatants revealed that wild-type <jats:italic>B. pseudomallei<\/jats:italic> and the <jats:italic>luxI<\/jats:italic> mutants synthesized numerous signalling molecules, including <jats:italic>N<\/jats:italic>-octanoyl-homoserine lactone, <jats:italic>N<\/jats:italic>-decanoyl-homoserine lactone, <jats:italic>N<\/jats:italic>-(3-hydroxyoctanoyl)-<jats:sc>l<\/jats:sc>-homoserine lactone, <jats:italic>N<\/jats:italic>-(3-hydroxydecanoyl)-<jats:sc>l<\/jats:sc>-homoserine lactone and <jats:italic>N<\/jats:italic>-(3-oxotetradecanoyl)-<jats:sc>l<\/jats:sc>-homoserine lactone, which was further confirmed by heterologous expression of the <jats:italic>B. pseudomallei luxI<\/jats:italic> alleles in <jats:italic>Escherichia coli<\/jats:italic>. Mutagenesis of the <jats:italic>B. pseudomallei<\/jats:italic> QS system increased the time to death and reduced organ colonization of aerosolized BALB\/c mice. Further, intraperitoneal challenge of Syrian hamsters with the <jats:italic>B. pseudomallei<\/jats:italic> QS mutants resulted in a significant increase in the LD<jats:sub>50.<\/jats:sub> Using semi-quantitative plate assays, preliminary analysis suggests that QS does not affect lipase, protease and phospholipase C biosynthesis\/secretion in <jats:italic>B. pseudomallei<\/jats:italic>. The findings of the investigation demonstrate that <jats:italic>B. pseudomallei<\/jats:italic> encodes multiple <jats:italic>luxIR<\/jats:italic> genes, and disruption of the QS alleles reduces animal pathogenicity, but does not affect exoproduct secretion.<\/jats:p>","DOI":"10.1099\/jmm.0.45661-0","type":"journal-article","created":{"date-parts":[[2004,10,20]],"date-time":"2004-10-20T23:09:52Z","timestamp":1098313792000},"page":"1053-1064","source":"Crossref","is-referenced-by-count":99,"title":["Role of quorum sensing in the pathogenicity of Burkholderia pseudomallei"],"prefix":"10.1099","volume":"53","author":[{"given":"Ricky L","family":"Ulrich","sequence":"first","affiliation":[{"name":"Bacteriology Division1 and Toxinology\/Aerobiology Division2, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD 21702-5011, USA 3Microbiology, Dstl, CBS Porton Down, Salisbury SP4 0JQ, UK 4Orion Genomics, Center for Emerging Technologies, 4041 Forest Park, St. Louis, MO 63108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"DeShazer","sequence":"additional","affiliation":[{"name":"Bacteriology Division1 and Toxinology\/Aerobiology Division2, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD 21702-5011, USA 3Microbiology, Dstl, CBS Porton Down, Salisbury SP4 0JQ, UK 4Orion Genomics, Center for Emerging Technologies, 4041 Forest Park, St. Louis, MO 63108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernst E","family":"Brueggemann","sequence":"additional","affiliation":[{"name":"Bacteriology Division1 and Toxinology\/Aerobiology Division2, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD 21702-5011, USA 3Microbiology, Dstl, CBS Porton Down, Salisbury SP4 0JQ, UK 4Orion Genomics, Center for Emerging Technologies, 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