{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T12:54:35Z","timestamp":1761742475191},"reference-count":23,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,12,1]]},"abstract":"<jats:p>Between January 2000 and March 2001,<jats:italic>Streptococcus pneumoniae<\/jats:italic>were isolated from the blood of 56 patients admitted to a single district general hospital in the South-East of England. The serotype and antibiotic susceptibility were determined for all isolates and, for those resistant to erythromycin, the presence or absence of the<jats:italic>mef<\/jats:italic>(A) and<jats:italic>erm<\/jats:italic>(B) genes was determined by PCR. Multi-locus sequence typing, along with PFGE, was undertaken on all isolates resistant to penicillin or erythromycin and a group of antibiotic-susceptible isolates, to identify whether globally distributed pneumococcal clones, as described by the Pneumococcal Molecular Epidemiology Network (PMEN), were present in the study population. Three serotype 9V penicillin-resistant isolates were identified as belonging to the Spain<jats:sup>9V<\/jats:sup>-3 clone, while 14 erythromycin-resistant isolates of serotype 14 belonged to the England<jats:sup>14<\/jats:sup>-9 clone. A single multi-resistant isolate of serotype 6B, was found to be a single-locus variant of the Spain<jats:sup>6B<\/jats:sup>-2 clone. All 14 erythromycin-resistant serotype 14 isolates possessed the<jats:italic>mef<\/jats:italic>(A) gene, while the single multi-resistant isolate possessed the<jats:italic>erm<\/jats:italic>(B) gene. These findings confirm the wide distribution and clinical impact of PMEN clones, which accounted for all of the penicillin and erythromycin resistance observed amongst invasive isolates in a district general hospital over a 15-month period.<\/jats:p>","DOI":"10.1099\/jmm.0.45763-0","type":"journal-article","created":{"date-parts":[[2004,11,19]],"date-time":"2004-11-19T19:48:14Z","timestamp":1100893694000},"page":"1241-1246","source":"Crossref","is-referenced-by-count":5,"title":["Antimicrobial resistance of invasive Streptococcus pneumoniae isolates in a British district general hospital: the international connection"],"prefix":"10.1099","volume":"53","author":[{"given":"Andrew","family":"Birtles","sequence":"first","affiliation":[{"name":"Respiratory and Systemic Infection Laboratory1 and Antibiotic Resistance Monitoring and Reference Laboratory3, Specialist and Reference Microbiology Division, Health Protection Agency, London NW9 5HT, UK 2Reading Public Health Laboratory, London 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5HT, UK 2Reading Public Health Laboratory, London Road, Reading RG1 5AN, UK 4Department of Biological Sciences, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel A","family":"Walker","sequence":"additional","affiliation":[{"name":"Respiratory and Systemic Infection Laboratory1 and Antibiotic Resistance Monitoring and Reference Laboratory3, Specialist and Reference Microbiology Division, Health Protection Agency, London NW9 5HT, UK 2Reading Public Health Laboratory, London Road, Reading RG1 5AN, UK 4Department of Biological Sciences, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan P","family":"Johnson","sequence":"additional","affiliation":[{"name":"Respiratory and Systemic Infection Laboratory1 and Antibiotic Resistance Monitoring and Reference Laboratory3, Specialist and Reference Microbiology 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