{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:28:06Z","timestamp":1788848886723,"version":"build-2803163510"},"reference-count":23,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,5,1]]},"abstract":"<jats:p>The transmission of<jats:italic>Brucella canis<\/jats:italic>to man commonly occurs through contact with infected dogs or their secretions, or through direct laboratory exposure. The disease is underdiagnosed due to a general lack of serological testing facilities and misconceptions concerning its prevalence. This report shows the potential use of an indirect ELISA (IELISA) for the diagnosis of human brucellosis caused by<jats:italic>B. canis<\/jats:italic>in a population of patients negative by smooth-<jats:italic>Brucella<\/jats:italic>antigen tests but positive by rapid slide agglutination test (RSAT). One hundred and ten sera from asymptomatic people found negative by tests using smooth<jats:italic>Brucella abortus<\/jats:italic>antigen and by RSAT showed an IELISA specificity of 100 % when a cut-off value of 27 % positivity (%P) was selected. For 17 sera from patients with positive<jats:italic>B. canis<\/jats:italic>culture or in close contact with culture-positive dogs, the IELISA sensitivity was 100 % with the same cut-off value. The positive patients presented clinical symptoms similar to brucellosis caused by other species of<jats:italic>Brucella<\/jats:italic>and some of them received antibiotic treatment and made good progress. Using this cut-off value, we studied 35 patients with negative blood cultures but positive RSATs, and IELISA detected 18 as positive; of the 17 IELISA-negative, two were RSAT-positive at dilution 1 : 2 and 15 were weakly positive with pure serum. These samples were probably from patients at an early stage of infection or indicate false-positive results. No cross-reaction was observed among the sera from nine cases with a diagnosis other than brucellosis, but cross-reactivity was evident in sera from patients infected with smooth-<jats:italic>Brucella<\/jats:italic>species. Since routine brucellosis diagnosis does not include<jats:italic>B. canis<\/jats:italic>investigation, infection with this species may be more widespread than is currently suspected. The RSAT could be a suitable screening test for the diagnosis of<jats:italic>B. canis<\/jats:italic>human brucellosis, and a supplementary technique, such as IELISA, performed on all positive RSAT samples that were negative by<jats:italic>B. abortus<\/jats:italic>antigen could ensure diagnostic specificity and confirm the diagnosis.<\/jats:p>","DOI":"10.1099\/jmm.0.45927-0","type":"journal-article","created":{"date-parts":[[2005,4,11]],"date-time":"2005-04-11T23:38:02Z","timestamp":1113262682000},"page":"457-461","source":"Crossref","is-referenced-by-count":74,"title":["Diagnosis of human brucellosis caused by Brucella canis"],"prefix":"10.1099","volume":"54","author":[{"given":"Nidia E","family":"Lucero","sequence":"first","affiliation":[{"name":"Brucellosis Laboratory, ANLIS Dr C. G. Malbr\u00e1n, Avda. Velez Sarsfield 563, 1281 Buenos Aires, Argentina 2Infectolog\u00eda, Hospital Cosme Argerich, Alte. Brown 240, 1155 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela I","family":"Escobar","sequence":"additional","affiliation":[{"name":"Brucellosis Laboratory, ANLIS Dr C. G. Malbr\u00e1n, Avda. Velez Sarsfield 563, 1281 Buenos Aires, Argentina 2Infectolog\u00eda, Hospital Cosme Argerich, Alte. Brown 240, 1155 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra M","family":"Ayala","sequence":"additional","affiliation":[{"name":"Brucellosis Laboratory, ANLIS Dr C. G. Malbr\u00e1n, Avda. Velez Sarsfield 563, 1281 Buenos Aires, Argentina 2Infectolog\u00eda, Hospital Cosme Argerich, Alte. Brown 240, 1155 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nestor","family":"Jacob","sequence":"additional","affiliation":[{"name":"Brucellosis Laboratory, ANLIS Dr C. G. Malbr\u00e1n, Avda. Velez Sarsfield 563, 1281 Buenos Aires, Argentina 2Infectolog\u00eda, Hospital Cosme Argerich, Alte. Brown 240, 1155 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"H1-7","first-page":"3","article-title":"A rapid slide agglutination test for the serodiagnosis of Brucella canis infection that employs a variant (M\u2212) organism as antigen","volume":"77","author":"Carmichael","year":"1987","journal-title":"Cornell Vet"},{"key":"H2-7","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1096-2867(96)80028-4","article-title":"Canine brucellosis: a diagnostician's dilemma","volume":"11","author":"Carmichael","year":"1996","journal-title":"Semin Vet Med Surg (Small Anim)"},{"key":"H3-7","article-title":"Brucellosis caused by Brucella canis: an Update of Infection in Animals and in Humans","author":"Carmichael","year":"1980"},{"key":"H4-7","first-page":"927","article-title":"Recent advances in the study of Brucella antigens and serological cross reactions","volume":"55","author":"Corbel","year":"1985","journal-title":"Vet Bull"},{"issue":"1","key":"H5-7","first-page":"377","article-title":"Genus Brucella , Meyer and Shaw 1920, 173AL","volume-title":"In Bergey's Manual of Systematic Bacteriology","volume":"vol","author":"Corbel","year":"1984"},{"key":"H6-7","first-page":"347","article-title":"A serological and bacteriological survey of canine brucellosis in Mexico","volume":"66","author":"Flores-Castro","year":"1976","journal-title":"Cornell Vet"},{"key":"H7-7","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1093\/oxfordjournals.aje.a112006","article-title":"Canine brucellosis in Florida: serological survey of pound dogs, animal shelter workers and veterinarians","volume":"100","author":"Hoff","year":"1974","journal-title":"Am J Epidemiol"},{"key":"H8-7","doi-asserted-by":"crossref","first-page":"157","DOI":"10.4269\/ajtmh.1975.24.157","article-title":"Serologic survey for agglutinins to Brucella canis in Florida residents","volume":"24","author":"Hoff","year":"1975","journal-title":"Am J Trop Med Hyg"},{"key":"H9-7","doi-asserted-by":"crossref","first-page":"204","DOI":"10.2105\/AJPH.63.3.204","article-title":"The incidence of Brucella canis antibodies in sera of military recruits","volume":"63","author":"Lewis","year":"1973","journal-title":"Am J Public Health"},{"key":"H10-7","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1128\/JCM.36.5.1425-1427.1998","article-title":"Buffered plate antigen test as a screening test for diagnosis of human brucellosis","volume":"36","author":"Lucero","year":"1998","journal-title":"J Clin Microbiol"},{"key":"H11-7","doi-asserted-by":"crossref","first-page":"3245","DOI":"10.1128\/JCM.37.10.3245-3248.1999","article-title":"Competitive enzyme immunoassay for diagnosis of human brucellosis","volume":"37","author":"Lucero","year":"1999","journal-title":"J Clin Microbiol"},{"key":"H12-7","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1099\/0022-1317-51-8-656","article-title":"Sensitivity and specificity of an indirect enzyme-linked immunoassay for the diagnosis of Brucella canis infection in dogs","volume":"51","author":"Lucero","year":"2002","journal-title":"J Med Microbiol"},{"key":"H13-7","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1007\/s100169900240","article-title":"Mycotic aneurysms of the tibioperoneal arteries","volume":"13","author":"McKee","year":"1999","journal-title":"Ann Vasc Surg"},{"key":"H14-7","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1128\/jcm.2.5.382-386.1975","article-title":"Seroepidemiological investigation of Brucella canis antibodies in different human population groups","volume":"2","author":"Monroe","year":"1975","journal-title":"J Clin Microbiol"},{"key":"H15-7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.vetmic.2003.12.010","article-title":"Serological relationship between cattle exposed to Brucella abortus , Yersinia enterocolitica O : 9 and Escherichia coli O157 : H7","volume":"100","author":"Nielsen","year":"2004","journal-title":"Vet Microbiol"},{"key":"H16-7","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/s002470050760","article-title":"Brucellosis: unusual presentations in two adolescent boys","volume":"30","author":"Piampiano","year":"2000","journal-title":"Pediatr Radiol"},{"key":"H17-7","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1093\/ajcp\/77.6.740","article-title":"A microagglutination test for human Brucella canis antibodies","volume":"77","author":"Polt","year":"1982","journal-title":"Am J Clin Pathol"},{"issue":"249","key":"H18-7","article-title":"Brucella canis infection in a woman with fever of unknown origin","volume":"78","author":"Rousseau","year":"1985","journal-title":"Postgrad Med"},{"key":"H19-7","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1055\/s-2008-1068393","article-title":"Brucella canis infection in man","volume":"111","author":"Schoenemann","year":"1986","journal-title":"Dtsch Med Wochenschr"},{"key":"H20-7","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0169-2607(95)01703-8","article-title":"MedCalc: a new computer program for medical statistics","volume":"48","author":"Schoonjans","year":"1995","journal-title":"Comput Methods Programs Biomed"},{"key":"H21-7","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/S0022-3476(82)80301-6","article-title":"Brucella canis infection in a 17-month-old child successfully treated with moxalactam","volume":"101","author":"Tosi","year":"1982","journal-title":"J Pediatr"},{"key":"H22-7","doi-asserted-by":"crossref","first-page":"110","DOI":"10.4269\/ajtmh.1979.28.110","article-title":"Occurrence of antibodies to Brucella canis in rural inhabitants of Corrientes and Neuqu\u00e9n provinces, Argentina","volume":"28","author":"Varela-Diaz","year":"1979","journal-title":"Am J Trop Med Hyg"},{"key":"H23-7","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1086\/313545","article-title":"Brucella canis endocarditis: case report","volume":"29","author":"Ying","year":"1999","journal-title":"Clin Infect Dis"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.45927-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T07:11:19Z","timestamp":1625728279000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.45927-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,5,1]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2005,5,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.45927-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,5,1]]}}}