{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T21:02:16Z","timestamp":1770411736295,"version":"3.49.0"},"reference-count":17,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,11,1]]},"abstract":"<jats:p>In this study penicillin-binding proteins (PBPs) of <jats:italic>Bacteroides fragilis<\/jats:italic> and the resistance mechanisms of this micro-organism to 11 \u03b2-lactam antibiotics were analysed. The study focused on the role of PBP2Bfr and metallo-\u03b2-lactamase in the mechanism of resistance to imipenem. The mechanism of \u03b2-lactam resistance in <jats:italic>B. fragilis<\/jats:italic> was strain dependent. The gene encoding the orthologue of <jats:italic>Escherichia coli<\/jats:italic> PBP3 gene (<jats:italic>pbpBBfr<\/jats:italic>, which encodes the protein PBP2Bfr) was sequenced in five of the eight strains studied, along with the <jats:italic>ccrA<\/jats:italic> (<jats:italic>cfiA<\/jats:italic>) gene in strain 119, and their implications for resistance were examined. Differences were found in the amino-acid sequence of PBP2Bfr in strains AK-2 and 119, and the production of \u03b2-lactamases indicated that these differences may be involved in the mechanism of resistance to imipenem. <jats:italic>In vitro<\/jats:italic> binding competition assays with membrane extracts using imipenem indicated that the PBP that bound imipenem with the highest affinity was PBP2Bfr, and that increased affinity in strain 7160 may be responsible for the moderate susceptibility of this strain to imipenem. In the same way, the importance of the chromosomal class A \u03b2-lactamase CepA in the resistance mechanism of the <jats:italic>B. fragilis<\/jats:italic> strains NCTC 9344, 7160, 2013E, AK-4, 0423 and R-212 was studied. In these strains this is the principal resistance mechanism to antimicrobial agents studied other than imipenem.<\/jats:p>","DOI":"10.1099\/jmm.0.45930-0","type":"journal-article","created":{"date-parts":[[2005,9,28]],"date-time":"2005-09-28T20:02:39Z","timestamp":1127937759000},"page":"1055-1064","source":"Crossref","is-referenced-by-count":31,"title":["Penicillin-binding proteins of Bacteroides fragilis and their role in the resistance to imipenem of clinical isolates"],"prefix":"10.1099","volume":"54","author":[{"given":"Juan","family":"Ayala","sequence":"first","affiliation":[{"name":"`\u2018Severo Ochoa\u2019\u2019 Molecular Biology Centre, CSIC-UAM, 28049 Cantoblanco, Madrid, Spain 2,3Biochemistry, Molecular Biology and Genetics Department2, and Medicine and Animal Health Department3, University of Extremadura, 10071 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Quesada","sequence":"additional","affiliation":[{"name":"`\u2018Severo Ochoa\u2019\u2019 Molecular Biology Centre, CSIC-UAM, 28049 Cantoblanco, Madrid, Spain 2,3Biochemistry, Molecular Biology and Genetics Department2, and Medicine and Animal Health Department3, University of Extremadura, 10071 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santiago","family":"Vadillo","sequence":"additional","affiliation":[{"name":"`\u2018Severo Ochoa\u2019\u2019 Molecular Biology Centre, CSIC-UAM, 28049 Cantoblanco, Madrid, Spain 2,3Biochemistry, Molecular Biology and Genetics Department2, and Medicine and Animal Health Department3, University of Extremadura, 10071 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jer\u00f3nimo","family":"Criado","sequence":"additional","affiliation":[{"name":"`\u2018Severo Ochoa\u2019\u2019 Molecular Biology Centre, CSIC-UAM, 28049 Cantoblanco, Madrid, Spain 2,3Biochemistry, Molecular Biology and Genetics Department2, and Medicine and Animal Health Department3, University of Extremadura, 10071 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Segundo","family":"P\u00edriz","sequence":"additional","affiliation":[{"name":"`\u2018Severo Ochoa\u2019\u2019 Molecular Biology Centre, CSIC-UAM, 28049 Cantoblanco, Madrid, Spain 2,3Biochemistry, Molecular Biology and Genetics Department2, and Medicine and Animal Health Department3, University of Extremadura, 10071 C\u00e1ceres, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"H1-9","doi-asserted-by":"crossref","first-page":"3032","DOI":"10.1128\/JCM.40.8.3032-3034.2002","article-title":"Cross-infection due to imipenem-resistant Bacteroides fragilis associated with a totally implantable venous port","volume":"40","author":"Arpin","year":"2002","journal-title":"J Clin Microbiol"},{"key":"H2-9","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1073\/pnas.91.6.2216","article-title":"PCR amplifications of up to 35-kb DNA with high fidelity and high yield from \u03bb bacteriophage templates","volume":"91","author":"Barnes","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"key":"H3-9","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1128\/AAC.30.5.645","article-title":"\u03b2-Lactamase mediated imipenem resistance in Bacteroides fragilis","volume":"30","author":"Cuchural","year":"1986","journal-title":"Antimicrob Agents Chemother"},{"key":"H4-9","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1099\/00222615-46-12-979","article-title":"Resistance to \u03b2-lactam antibiotics in Bacteroides spp","volume":"46","author":"Edwards","year":"1997","journal-title":"J Med Microbiol"},{"key":"H5-9","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1093\/jac\/38.6.941","article-title":"Mechanisms responsible for reduced susceptibility to imipenem in Bacteroides fragilis","volume":"38","author":"Edwards","year":"1996","journal-title":"J Antimicrob Chemother"},{"key":"H6-9","doi-asserted-by":"crossref","first-page":"907","DOI":"10.7164\/antibiotics.36.907","article-title":"Penicillin-binding proteins in Bacteroides fragilis","volume":"36","author":"Georgopapadakou","year":"1983","journal-title":"J Antibiot"},{"key":"H7-9","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1128\/AAC.47.3.979-985.2003","article-title":"New insertion sequence elements in the upstream region of cfiA in imipenem-resistant Bacteroides fragilis strains","volume":"47","author":"Kato","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"H8-9","volume-title":"Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. 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