{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:59:43Z","timestamp":1791349183122,"version":"4.3.1"},"reference-count":28,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,11,1]]},"abstract":"\n Microbiota in gut contents of jejunum, ileum, caecum and recto-sigmoid colon obtained from three elderly individuals at autopsy were compared using 16S rRNA gene libraries and terminal restriction fragment length polymorphism (T-RFLP). Random clones of 16S rRNA gene sequences were isolated after PCR amplification with universal primer sets of total genomic DNA extracted from each sample of gut contents. An average of 90 randomly selected clones were partially sequenced (about 500 bp). T-RFLP analysis was performed using the 16S rRNA gene amplified from each sample. The lengths of the terminal restriction fragments were analysed after digestion with\n Hha<\/jats:italic>\n I and\n Msp<\/jats:italic>\n I. The jejunal and ileal microbiota consisted of simple microbial communities of streptococci, lactobacilli, \u2018\n Gammaproteobacteria<\/jats:italic>\n ', the\n Enterococcus<\/jats:italic>\n group and the\n Bacteroides<\/jats:italic>\n group. Most of the species were facultative anaerobes or aerobes. The\n Clostridium coccoides<\/jats:italic>\n group and the\n Clostridium leptum<\/jats:italic>\n subgroup, which are the most predominant groups in human faeces, were not detected in samples from the upper gastrointestinal tract. The caecal microbiota was more complex than the jejunal and ileal microbiota. The\n C<\/jats:italic>\n .\n coccoides<\/jats:italic>\n group, the\n C<\/jats:italic>\n .\n leptum<\/jats:italic>\n subgroup and the\n Bacteroides<\/jats:italic>\n group were detected in the caecum. The recto-sigmoidal colonic microbiota consisted of complex microbial communities, with numerous species that belonged to the\n C. coccoides<\/jats:italic>\n group, the\n C. leptum<\/jats:italic>\n subgroup, the\n Bacteroides<\/jats:italic>\n group, \u2018\n Gammaproteobacteria<\/jats:italic>\n ', the\n Bifidobacterium<\/jats:italic>\n group, streptococci and lactobacilli, and included more than 26 operational taxonomic units. The results showed marked individual differences in the composition of microbiota in each region.\n <\/jats:p>","DOI":"10.1099\/jmm.0.45935-0","type":"journal-article","created":{"date-parts":[[2005,9,28]],"date-time":"2005-09-28T16:02:39Z","timestamp":1127923359000},"page":"1093-1101","source":"Crossref","is-referenced-by-count":311,"title":["Molecular analysis of jejunal, ileal, caecal and recto-sigmoidal human colonic microbiota using 16S rRNA gene libraries and terminal restriction fragment length polymorphism"],"prefix":"10.1099","volume":"54","author":[{"given":"Hidenori","family":"Hayashi","sequence":"first","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 2Departments of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3Department of Medicine, Shiga Health Insurance Hospital, Otsu, Shiga 520-0846, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rei","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 2Departments of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3Department of Medicine, Shiga Health Insurance Hospital, Otsu, Shiga 520-0846, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Nishi","sequence":"additional","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 2Departments of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3Department of Medicine, Shiga Health Insurance Hospital, Otsu, Shiga 520-0846, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuo","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 2Departments of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3Department of Medicine, Shiga Health Insurance Hospital, Otsu, Shiga 520-0846, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimi","family":"Benno","sequence":"additional","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351-0198, Japan 2Departments of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan 3Department of Medicine, Shiga Health Insurance Hospital, Otsu, Shiga 520-0846, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"H1-14","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1128\/AEM.55.5.1100-1105.1989","article-title":"Comparison of fecal microflora of elderly persons in rural and urban areas of Japan","volume":"55","author":"Benno","year":"1989","journal-title":"Appl Environ Microbiol"},{"key":"H2-14","first-page":"421","article-title":"The microflora of the human ileum and interabdominal colon: results of direct needle aspiration at surgery and evaluation of the technique","volume":"79","author":"Bentley","year":"1972","journal-title":"J Lab Clin Med"},{"key":"H3-14","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1093\/nar\/gkg039","article-title":"& 8 other authors","volume":"31","author":"Cole","year":"2003","journal-title":"Nucleic Acids Res"},{"key":"H4-14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/infdis\/137.1.1","article-title":"Bacterial flora of the small bowel before and after bypass procedure for morbid obesity","volume":"137","author":"Corrodi","year":"1978","journal-title":"J Infect Dis"},{"key":"H5-14","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/B978-0-12-341280-5.50007-0","article-title":"Normal indigenous intestinal flora","volume-title":"In Human Intestinal Microflora in Health and Disease","author":"Finegold","year":"1983"},{"key":"H6-14","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1093\/biomet\/40.3-4.237","article-title":"The population frequencies of species and the estimation of population parameters","volume":"40","author":"Good","year":"1953","journal-title":"Biometrica"},{"key":"H7-14","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/S0016-5085(19)34122-8","article-title":"Studies of intestinal microflora.II. 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