{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T08:24:09Z","timestamp":1759047849804},"reference-count":28,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,11,1]]},"abstract":"<jats:p>An evaluation of the microbiota present in cutaneous ulcers from 31 patients with a clinical and parasitological diagnosis of American tegumentary leishmaniasis (ATL) was carried out by the standard filter paper disc technique, including antimicrobial susceptibility of the bacterial isolates. Microbial examination indicated that 21 patients (67.7 %) were contaminated with one to four bacteria and some of them also with yeast. A total of 142 micro-organisms were isolated.<jats:italic>Staphylococcus aureus<\/jats:italic>was the most frequently recovered bacterium (95.2 % of positive patients) and was found to produce type B (70 % of the staphylococcal isolates) and type C (50 %) enterotoxins as well as toxic shock syndrome toxin (60 %).<jats:italic>Proteus mirabilis<\/jats:italic>(33.3 % of the positive patients),<jats:italic>Streptococcus pyogenes<\/jats:italic>(19.0 %), H<jats:sub>2<\/jats:sub>S-negative<jats:italic>Proteus<\/jats:italic>species (19.0 %),<jats:italic>Klebsiella oxytoca<\/jats:italic>(14.3 %),<jats:italic>Enterobacter<\/jats:italic>species (9.5 %),<jats:italic>Peptostreptococcus<\/jats:italic>species (9.5 %),<jats:italic>Pseudomonas<\/jats:italic>species (4.8 %),<jats:italic>Prevotella bivia<\/jats:italic>(4.8 %),<jats:italic>Escherichia coli<\/jats:italic>(4.8 %),<jats:italic>Streptococcus agalactiae<\/jats:italic>(4.8 %),<jats:italic>Bacteroides fragilis<\/jats:italic>(4.8 %),<jats:italic>Candida albicans<\/jats:italic>(9.5 %) and<jats:italic>Candida tropicalis<\/jats:italic>(4.8 %) were also isolated. Surprisingly,<jats:italic>Staph. aureus<\/jats:italic>isolates were susceptible to almost all tested drugs, although some of them were resistant to penicillin (69 %) and ampicillin + sulbactam (68 %). Concerning obligate anaerobes, all the Gram-negative isolates (25 % of the total) were resistant to metronidazole. The results of the present study show that microbial secondary contaminants, particularly<jats:italic>Staph. aureus<\/jats:italic>, should be considered in the diagnosis and treatment of ATL lesions.<\/jats:p>","DOI":"10.1099\/jmm.0.46070-0","type":"journal-article","created":{"date-parts":[[2005,9,28]],"date-time":"2005-09-28T20:02:39Z","timestamp":1127937759000},"page":"1071-1076","source":"Crossref","is-referenced-by-count":17,"title":["Identification and antimicrobial susceptibility of micro-organisms recovered from cutaneous lesions of human American tegumentary leishmaniasis in Minas Gerais, Brazil"],"prefix":"10.1099","volume":"54","author":[{"given":"Cla\u00fadia O","family":"Fontes","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia1 and Departamento de Parasitologia2, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, Minas Gerais, Brazil 3Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Auxiliadora R","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia1 and Departamento de Parasitologia2, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, Minas Gerais, Brazil 3Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques R","family":"Nicoli","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia1 and Departamento de Parasitologia2, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, Minas Gerais, Brazil 3Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junia S","family":"Hamdan","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia1 and Departamento de Parasitologia2, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, Minas Gerais, Brazil 3Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilson","family":"Mayrink","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia1 and Departamento de Parasitologia2, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, Minas Gerais, Brazil 3Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odair","family":"Genaro","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia1 and Departamento de Parasitologia2, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, Minas Gerais, Brazil 3Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz S","family":"Carmo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia1 and Departamento de Parasitologia2, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, Minas Gerais, Brazil 3Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz M","family":"Farias","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia1 and Departamento de Parasitologia2, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, Minas Gerais, Brazil 3Funda\u00e7\u00e3o Ezequiel Dias, Belo Horizonte, 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