{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:36:16Z","timestamp":1772156176765,"version":"3.50.1"},"reference-count":11,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,1,1]]},"abstract":"\n Rapid detection of micro-organisms from blood is one of the most critical functions of a diagnostic microbiology laboratory. Automated blood-culture systems reduce the time needed to detect positive cultures, and reduce specimen handling. The false-positive rate of such systems is 1\u201310\u200a%. In this study, the presence of pathogens in \u2018false-positive\u2019 bottles obtained from BACTEC 9050 (Becton Dickinson) and BacT\/Alert (Biom\u00e9rieux) systems was investigated by eubacterial and fungal PCR. A total of 169 subculture-negative aerobic blood-culture bottles (104 BacT\/Alert and 65 BACTEC) were evaluated. Both fungal and eubacterial PCRs were negative for all BACTEC bottles. Fungal PCR was also negative for the BacT\/Alert system, but 10 bottles (9\u00b76\u200a%) gave positive results by eubacterial PCR. Sequence analysis of the positive PCR amplicons indicated the presence of the following bacteria (number of isolates in parentheses):\n Pasteurella multocida<\/jats:italic>\n (1), S\n taphylococcus epidermidis<\/jats:italic>\n (2),\n Staphylococcus hominis<\/jats:italic>\n (1),\n Micrococcus<\/jats:italic>\n sp. (1), S\n treptococcus pneumoniae<\/jats:italic>\n (1),\n Corynebacterium<\/jats:italic>\n spp. (2),\n Brachibacterium<\/jats:italic>\n sp. (1) and\n Arthrobacter\/Rothia<\/jats:italic>\n sp. (1). Antibiotic usage by the patients may be responsible for the inability of the laboratory to grow these bacteria on subcultures. For patients with more than one false-positive bottle, molecular methods can be used to evaluate the microbial DNA in these bottles. False positives from the BACTEC system may be due to elevated patient leukocyte counts or the high sensitivity of the system to background increases in CO\n 2<\/jats:sub>\n concentration.\n <\/jats:p>","DOI":"10.1099\/jmm.0.46196-0","type":"journal-article","created":{"date-parts":[[2005,12,30]],"date-time":"2005-12-30T16:02:36Z","timestamp":1135958556000},"page":"53-57","source":"Crossref","is-referenced-by-count":26,"title":["PCR evaluation of false-positive signals from two automated blood-culture systems"],"prefix":"10.1099","volume":"55","author":[{"given":"Z. Ceren","family":"Karahan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ipek","family":"Mumcuoglu","sequence":"additional","affiliation":[{"name":"Microbiology and Clinical Microbiology Department, Ankara Numune Education and Investigation Hospital, 06100-Sihhiye, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haluk","family":"Guriz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deniz","family":"Tamer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neriman","family":"Balaban","sequence":"additional","affiliation":[{"name":"Microbiology and Clinical Microbiology Department, Ankara Numune Education and Investigation Hospital, 06100-Sihhiye, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derya","family":"Aysev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nejat","family":"Akar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1128\/JCM.35.6.1469-1472.1997","article-title":"& 7 other authors","volume":"35","author":"Cockerill","year":"1997","journal-title":"J Clin Microbiol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.diagmicrobio.2003.08.001","article-title":"Verification of false-positive blood culture results generated by the BACTEC 9000 series by eubacterial 16S rDNA and panfungal 18S rDNA directed polymerase chain reaction (PCR)","volume":"48","author":"Daxboeck","year":"2004","journal-title":"Diagn Microbiol Infect 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Methods"},{"key":"R6","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1128\/JCM.31.3.552-557.1993","article-title":"Multicenter clinical evaluation of continuous monitoring blood culture system using fluorescent-sensor technology","volume":"31","author":"Nolte","year":"1993","journal-title":"J Clin Microbiol"},{"key":"R7","doi-asserted-by":"crossref","first-page":"3578","DOI":"10.1128\/JCM.39.10.3578-3582.2001","article-title":"& 7 other authors","volume":"39","author":"Qian","year":"2001","journal-title":"J Clin Microbiol"},{"key":"R8","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/s10156-004-0349-8","article-title":"Can BacT\/Alert FA and FN blood culture bottles increase the recovery of microorganisms in the clinical laboratory?","volume":"10","author":"Saito","year":"2004","journal-title":"J Chemother"},{"key":"R9","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1128\/JCM.33.7.1905-1908.1995","article-title":"Comparison of BACTEC 9240 and BacT\/Alert blood culture systems in an adult hospital","volume":"33","author":"Smith","year":"1995","journal-title":"J Clin Microbiol"},{"key":"R10","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/S0928-8244(98)00150-3","article-title":"Identification of medically significant fungal genera by polymerase chain reaction followed by restriction enzyme analysis","volume":"23","author":"Velegraki","year":"1999","journal-title":"FEMS Immunol Med Microbiol"},{"key":"R11","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/JCM.36.3.657-661.1998","article-title":"Controlled clinical laboratory comparison of two supplemented aerobic and anaerobic media used in automated blood culture systems to detect bloodstream infections","volume":"36","author":"Ziegler","year":"1998","journal-title":"J Clin Microbiol"}],"container-title":["Journal of Medical 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