{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:04:14Z","timestamp":1791180254250,"version":"4.1.0"},"reference-count":31,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,2,1]]},"abstract":"<jats:p>Real-time PCR was employed to detect a region of the P1 cytadhesin gene of <jats:italic>Mycoplasma pneumoniae<\/jats:italic> in clinical samples. An internal processing control was included that could be co-amplified simultaneously in the same reaction tube. The assay could reproducibly detect 1\u00d710<jats:sup>3<\/jats:sup> \n                  <jats:italic>M. pneumoniae<\/jats:italic> organisms ml<jats:sup>\u22121<\/jats:sup> in clinical samples. There was no amplification of DNA or signal production from 15 other species of human mycoplasmas and 19 other bacterial species. Using a panel of 175 respiratory samples taken from patients with pneumonia of proven aetiology, the sensitivity was found to be 60\u200a% and the specificity of the assay 96\u00b77\u200a% when compared with serology. This assay is suitable for same-day diagnosis of <jats:italic>M. pneumoniae<\/jats:italic> infection and batch processing of respiratory samples for clinical screening.<\/jats:p>","DOI":"10.1099\/jmm.0.46281-0","type":"journal-article","created":{"date-parts":[[2006,1,24]],"date-time":"2006-01-24T22:05:41Z","timestamp":1138140341000},"page":"149-155","source":"Crossref","is-referenced-by-count":46,"title":["Real-time detection of Mycoplasma pneumoniae in respiratory samples with an internal processing control"],"prefix":"10.1099","volume":"55","author":[{"given":"David","family":"Pitcher","sequence":"first","affiliation":[{"name":"Respiratory and Systemic Infection Laboratory, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria J.","family":"Chalker","sequence":"additional","affiliation":[{"name":"Respiratory and Systemic Infection Laboratory, Health Protection Agency Centre 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