{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T14:56:49Z","timestamp":1791903409681,"version":"4.3.4"},"reference-count":38,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,8,1]]},"abstract":"The distribution of 19 major virulence genes and the presence of plasmids were surveyed in 141 Legionella pneumophila<\/jats:italic> serogroup (SG) 1 isolates from patients and water in Queensland, Australia. The results showed that 16 of the virulence genes examined were present in all isolates, suggesting that they are life-essential genes for isolates in the environment and host cells. The 65\u2005kb pathogenicity island identified originally in strain Philadelphia-1T<\/jats:sup> was detected more frequently in isolates from water (44.2\u200a%) than in those from patients (2.7\u200a%), indicating that the 65\u2005kb DNA fragment may aid the survival of L. pneumophila<\/jats:italic> in the sampled environment. However, the low frequency of the 65\u2005kb fragment in isolates from patients suggests that the pathogenicity island may not be necessary for L. pneumophila<\/jats:italic> to cause disease. Plasmids were not detected in the L. pneumophila<\/jats:italic> SG1 isolates from patients or water studied. There was an association of both lvh<\/jats:italic> and rtxA<\/jats:italic> with the virulent and predominant genotype detected by amplified fragment length polymorphism, termed AF1, whereas the avirulent common isolate from water termed AF16 did not have lvh<\/jats:italic> or rtxA<\/jats:italic> genes, with the exception of one isolate with rtxA<\/jats:italic>. It was found that a PCR detection test strategy with lvh<\/jats:italic> and rtxA<\/jats:italic> as pathogenesis markers would be useful for determining the infection potential of an isolate.<\/jats:p>","DOI":"10.1099\/jmm.0.46310-0","type":"journal-article","created":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T23:08:08Z","timestamp":1153264088000},"page":"993-997","source":"Crossref","is-referenced-by-count":20,"title":["Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, Australia"],"prefix":"10.1099","volume":"55","author":[{"given":"Bixing","family":"Huang","sequence":"first","affiliation":[{"name":"Public Health Microbiology Laboratory, Queensland Health Scientific Services, 39 Kessels Road, Coopers Plains, QLD 4108, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Yuan","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience, 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