{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T02:09:09Z","timestamp":1768097349118,"version":"3.49.0"},"reference-count":32,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,5,1]]},"DOI":"10.1099\/jmm.0.46350-0","type":"journal-article","created":{"date-parts":[[2006,4,3]],"date-time":"2006-04-03T21:48:02Z","timestamp":1144100882000},"page":"551-559","source":"Crossref","is-referenced-by-count":29,"title":["Using real-time PCR to specifically detect Burkholderia mallei"],"prefix":"10.1099","volume":"55","author":[{"given":"Melanie P.","family":"Ulrich","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Norwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deanna R.","family":"Christensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricky L.","family":"Ulrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","first-page":"3","article-title":"Molecular-genetic approaches to diagnosis and intraspecific typing of causative agents of glanders and melioidosis","volume":"2","author":"Antonov","year":"2005","journal-title":"Mol Gen Mikrobiol Virusol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"2737","DOI":"10.1128\/JCM.36.9.2737-2741.1998","article-title":"Molecular procedure for rapid detection of Burkholderia mallei and Burkholderia pseudomallei","volume":"36","author":"Bauernfeind","year":"1998","journal-title":"J Clin Microbiol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1017\/S095026889600739X","article-title":"Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei -like strains","volume":"118","author":"Brett","year":"1997","journal-title":"Epidemiol Infect"},{"key":"R4","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1099\/00207713-48-1-317","article-title":"Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei -like species","volume":"48","author":"Brett","year":"1998","journal-title":"Int J Syst Bacteriol"},{"key":"R5","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1128\/JCM.32.11.2660-2666.1994","article-title":"Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis , Brucella ovis , and Brucella suis bv. 1 by PCR","volume":"32","author":"Bricker","year":"1994","journal-title":"J Clin Microbiol"},{"key":"R6","doi-asserted-by":"crossref","first-page":"4859","DOI":"10.1128\/JCM.42.10.4859-4862.2004","article-title":"Comparative analysis of the Schleicher and Schuell IsoCode Stix DNA isolation device and the Qiagen QIAamp DNA","volume":"42","author":"Coyne","year":"2004","journal-title":"Mini Kit. J Clin Microbiol"},{"key":"R7","first-page":"209","article-title":"Glanders: new insights into an old disease","volume-title":"In Biological Weapons Defense: Infectious Diseases and Counterbioterrorism","author":"DeShazer","year":"2004"},{"key":"R8","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1354\/vp.37-6-626","article-title":"Mouse model of sublethal and lethal intraperitoneal glanders ( Burkholderia mallei )","volume":"37","author":"Fritz","year":"2000","journal-title":"Vet Pathol"},{"key":"R9","doi-asserted-by":"crossref","first-page":"4647","DOI":"10.1128\/JCM.41.10.4647-4654.2003","article-title":"Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei","volume":"41","author":"Gee","year":"2003","journal-title":"J Clin Microbiol"},{"key":"R10","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1128\/JCM.43.1.479-483.2005","article-title":"Preliminary evaluation of the API 20NE and RapID NF plus systems for rapid identification of Burkholderia pseudomallei and B. mallei","volume":"43","author":"Glass","year":"2005","journal-title":"J Clin Microbiol"},{"key":"R11","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1128\/JCM.41.5.2068-2079.2003","article-title":"Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei","volume":"41","author":"Godoy","year":"2003","journal-title":"J Clin Microbiol"},{"key":"R12","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1136\/mp.55.6.398","article-title":"Strategies for PCR based detection of Burkholderia pseudomallei DNA in paraffin wax embedded tissues","volume":"55","author":"Hagen","year":"2002","journal-title":"Mol Pathol"},{"key":"R13","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.mcp.2004.07.006","article-title":"Development of a novel internal positive control for Taqman based assays","volume":"19","author":"Hartman","year":"2005","journal-title":"Mol Cell Probes"},{"key":"R14","doi-asserted-by":"crossref","first-page":"14240","DOI":"10.1073\/pnas.0403302101","article-title":"& 45 other authors","volume":"101","author":"Holden","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.7326\/0003-4819-26-1-93","article-title":"Human glanders: report of six cases","volume":"26","author":"Howe","year":"1947","journal-title":"Ann Intern Med"},{"key":"R16","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/S0140-6736(00)79078-7","article-title":"Saints and martyrs","volume":"231","author":"Hunter","year":"1936","journal-title":"Lancet"},{"key":"R17","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1080\/00313029800169685","article-title":"Potential misidentification of Burkholderia pseudomallei by API 20NE","volume":"30","author":"Inglis","year":"1998","journal-title":"Pathology"},{"key":"R18","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.femsim.2004.10.008","article-title":"Detection and differentiation of Burkholderia pseudomallei , Burkholderia mallei and Burkholderia thailandensis by multiplex PCR","volume":"43","author":"Lee","year":"2005","journal-title":"FEMS Immunol Med Microbiol"},{"key":"R19","doi-asserted-by":"crossref","first-page":"14246","DOI":"10.1073\/pnas.0403306101","article-title":"& 30 other authors","volume":"101","author":"Nierman","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R20","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1111\/j.1574-695X.2002.tb00629.x","article-title":"A possible pitfall in the identification of Burkholderia mallei using molecular identification systems based on the sequence of the flagellin fliC gene","volume":"34","author":"Sprague","year":"2002","journal-title":"FEMS Immunol Med Microbiol"},{"key":"R21","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1056\/NEJM200107263450404","article-title":"Glanders in a military research microbiologist","volume":"345","author":"Srinivasan","year":"2001","journal-title":"N Engl J Med"},{"key":"R22","first-page":"339","article-title":"Glanders","volume-title":"In CRC Handbook Series in Zoonoses","author":"Steele","year":"1979"},{"key":"R23","doi-asserted-by":"crossref","DOI":"10.1128\/JB.187.22.7857-7862.2005","article-title":"Actin-binding proteins from Burkholderia mallei and B. thailandensis can functionally compensate for the actin-based motility defect of a B. pseudomallei bimA mutant","author":"Stevens","year":"2005a","journal-title":"J Bacteriol"},{"key":"R24","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/j.1365-2958.2004.04528.x","article-title":"Identification of a bacterial factor required for actin-based motility of Burkholderia pseudomallei","volume":"56","author":"Stevens","year":"2005b","journal-title":"Mol Microbiol"},{"key":"R25","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.mcp.2003.09.010","article-title":"PCR-RFLP based differentiation of Burkholderia mallei and Burkholderia pseudomallei","volume":"18","author":"Tanpiboonsak","year":"2004","journal-title":"Mol Cell Probes"},{"key":"R26","doi-asserted-by":"crossref","first-page":"5871","DOI":"10.1128\/JCM.42.12.5871-5874.2004","article-title":"Identification and discrimination of Burkholderia pseudomallei , B. mallei , and B. thailandensis by real-time PCR targeting type III secretion system genes","volume":"42","author":"Thibault","year":"2004","journal-title":"J Clin Microbiol"},{"key":"R27","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1097\/01.pdm.0000137099.36618.cc","article-title":"Development of 5\u2032 nuclease real-time PCR assays for the rapid identification of the Burkholderia mallei \/\/ Burkholderia pseudomallei complex","volume":"13","author":"Tomaso","year":"2004","journal-title":"Diagn Mol Pathol"},{"key":"R28","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.mcp.2004.08.001","article-title":"Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probes","volume":"19","author":"Tomaso","year":"2005","journal-title":"Mol Cell Probes"},{"key":"R29","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1128\/CDLI.2.4.448-453.1995","article-title":"Oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16S\u201323S rRNA internal transcribed spacers","volume":"2","author":"Tyler","year":"1995","journal-title":"Clin Diagn Lab Immunol"},{"key":"R30","doi-asserted-by":"crossref","DOI":"10.1016\/j.diagmicrobio.2005.11.007","article-title":"Development of a PCR assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related Burkholderiaceae","author":"Ulrich","year":"2006","journal-title":"Diagn Microbiol Infect Dis"},{"key":"R31","doi-asserted-by":"crossref","first-page":"5771","DOI":"10.1128\/JCM.43.11.5771-5774.2005","article-title":"Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei","volume":"43","author":"U'Ren","year":"2005","journal-title":"J Clin Microbiol"},{"key":"R32","doi-asserted-by":"crossref","first-page":"4003","DOI":"10.1128\/JB.184.14.4003-4017.2002","article-title":"Burkholderia thailandensis E125 harbors a temperate bacteriophage specific for Burkholderia mallei","volume":"184","author":"Woods","year":"2002","journal-title":"J Bacteriol"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.46350-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T02:21:22Z","timestamp":1585880482000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.46350-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5,1]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,5,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.46350-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5,1]]}}}