{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T15:45:42Z","timestamp":1759160742653},"reference-count":24,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,9,1]]},"abstract":"In this study, the phenotypic and genotypic resistance to fluoroquinolones in Neisseria gonorrhoeae<\/jats:italic> isolated in Jiangsu Province, China, was analysed. In vitro<\/jats:italic> susceptibility testing of eight antimicrobial agents, including ciprofloxacin and levofloxacin, against 95 clinical isolates was carried out. Detection of mutations in the gyrA<\/jats:italic> and parC<\/jats:italic> genes was performed by sequence analysis. The clinical isolates demonstrated 100\u200a% resistance to ciprofloxacin and 98.9\u200a% non-susceptibility to levofloxacin. All of the isolates were susceptible to cefotaxime and ceftriaxone. For cefepime, spectinomycin and tetracycline, 98.9, 94.7 and 1.1\u200a% of the isolates were susceptible, respectively. None of the isolates was susceptible to penicillin. Five types based on gyrA<\/jats:italic> mutations could be categorized among 54 isolates with seven different mutation sites found on their parC<\/jats:italic> gene. Analysis of sequence results showed that the gyrA<\/jats:italic> mutation Asp-95\u2192Ala and the parC<\/jats:italic> mutations Ser-87\u2192Arg and Ser-87\u2192Asn made a significant contribution to the resistance to fluoroquinolones, in addition to double mutations found in each gene. Therefore, the use of fluoroquinolones in the treatment of N. gonorrhoeae<\/jats:italic> infections in Jiangsu Province is not recommended, while the use of third- and fourth-generation cephalosporins and spectinomycin is recommended.<\/jats:p>","DOI":"10.1099\/jmm.0.46401-0","type":"journal-article","created":{"date-parts":[[2006,8,16]],"date-time":"2006-08-16T18:42:50Z","timestamp":1155753770000},"page":"1251-1255","source":"Crossref","is-referenced-by-count":15,"title":["Antimicrobial susceptibility of Neisseria gonorrhoeae isolated in Jiangsu Province, China, with a focus on fluoroquinolone resistance"],"prefix":"10.1099","volume":"55","author":[{"given":"Bei","family":"Wang","sequence":"first","affiliation":[{"name":"School of Public Health, Southeast University, Nanjing, Jiangsu, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-shui","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Public Health, Southeast University, Nanjing, Jiangsu, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-xian","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health, Southeast University, Nanjing, Jiangsu, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zu-huang","family":"Mi","sequence":"additional","affiliation":[{"name":"Wuxi Clon-Gen Technique Institute, Wuxi, Jiangsu, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue-pu","family":"Pu","sequence":"additional","affiliation":[{"name":"School of Public Health, Southeast University, Nanjing, Jiangsu, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamie","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong Special Adminstrative Region, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas K. 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