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The 17\u2005kDa<jats:italic>A. actinomycetemcomitans<\/jats:italic>protein was identified as peptidoglycan-associated lipoprotein (PAL; AaPAL) by two-dimensional immunoblotting and subsequent sequence analysis by mass spectrometry and bioinformatics tools. AaPAL was an OMP and a lipoprotein, and it had an OmpA-like domain. In a group of middle-aged subjects (<jats:italic>n<\/jats:italic>=26), serum reactivity to AaPAL was associated with the presence of periodontitis but not with the oral detection of<jats:italic>A. actinomycetemcomitans<\/jats:italic>. Both human sera and rabbit antisera against three different types of antigens, the gel-purified AaPAL,<jats:italic>A. actinomycetemcomitans<\/jats:italic>whole-cell antigens, and CsgA, recognized putative PALs of oral haemophili in addition to AaPAL. The results demonstrated that the novel AaPAL is a conserved bacterial lipoprotein. It is expressed<jats:italic>in vivo<\/jats:italic>and is strongly immunoreactive. The antigenic cross-reactivity found between AaPAL and oral haemophili may enhance local and systemic immuno-inflammatory reactions in periodontitis.<\/jats:p>","DOI":"10.1099\/jmm.0.46470-0","type":"journal-article","created":{"date-parts":[[2006,6,13]],"date-time":"2006-06-13T16:53:51Z","timestamp":1150217631000},"page":"931-942","source":"Crossref","is-referenced-by-count":31,"title":["Immunoproteomics of Actinobacillus actinomycetemcomitans outer-membrane proteins reveal a highly immunoreactive peptidoglycan-associated lipoprotein"],"prefix":"10.1099","volume":"55","author":[{"given":"Maneesh","family":"Paul-Satyaseela","sequence":"first","affiliation":[{"name":"Oral Microbiology, Institute of Dentistry, Ume\u00e5 University, SE-90187 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maribasappa","family":"Karched","sequence":"additional","affiliation":[{"name":"Oral Microbiology, Institute of Dentistry, Ume\u00e5 University, SE-90187 Ume\u00e5, 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