{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T12:00:58Z","timestamp":1780142458363,"version":"3.54.0"},"reference-count":13,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,12,1]]},"abstract":"The objective of this study was to develop and evaluate a rapid new method of identifying clinically relevant Nocardia<\/jats:italic> species. DNA extracted from different Nocardia<\/jats:italic> strains was used in a real-time PCR assay with SYBR Green and melting-curve analysis to identify Nocardia<\/jats:italic> species. Ten control strains and four bacterial strains of closely related genera were employed, and samples from 28 patients were used. All Nocardia<\/jats:italic> strains were identified correctly, and there was no cross-reaction with strains from genera closely related to Nocardia<\/jats:italic>. The sensitivity and specificity of the method were 90 and 100\u200a%, respectively. This method can be used to rapidly detect Nocardia<\/jats:italic> species in culture, without cross-reaction with other closely related genera.<\/jats:p>","DOI":"10.1099\/jmm.0.46593-0","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T03:24:42Z","timestamp":1163647482000},"page":"1711-1715","source":"Crossref","is-referenced-by-count":19,"title":["Rapid identification of clinically relevant Nocardia species using real-time PCR with SYBR Green and melting-curve analysis"],"prefix":"10.1099","volume":"55","author":[{"given":"Mubarak","family":"Alfaresi","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine \u2013 Microbiology Division, Zayed Military Hospital, Abu Dhabi, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abida","family":"Elkosh","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine \u2013 Microbiology Division, Zayed Military Hospital, Abu Dhabi, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","volume-title":"Laboratory Methods for the Diagnosis of Nocardiosis","author":"Boiron","year":"1993"},{"key":"R2","first-page":"502","volume-title":"Nocardia , Rhodococcus , Gordonia , Actinomadura , Streptomyces , and other aerobic actinomycetes","author":"Brown","year":"2003"},{"key":"R3","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1097\/00019048-199901000-00005","article-title":"Nocardiosis: a clinical review","volume":"8","author":"Burgert","year":"1999","journal-title":"Infect Dis Clin Pract"},{"key":"R4","first-page":"1026","article-title":"Kinetic PCR analysis: real-time monitoring of DNA amplification reactions","volume":"( N Y ) 11","author":"Higuchi","year":"1993","journal-title":"Bio-Technology"},{"key":"R5","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1111\/j.1348-0421.1997.tb01878.x","article-title":"& 7 other authors","volume":"41","author":"Hou","year":"1997","journal-title":"Microbiol Immunol"},{"key":"R6","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1128\/JCM.37.1.99-102.1999","article-title":"Rapid identification of clinically relevant nocardia species to genus level by 16S rRNA gene PCR","volume":"37","author":"Laurent","year":"1999","journal-title":"J Clin Microbiol"},{"key":"R7","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1093\/clinids\/22.6.891","article-title":"Nocardiosis","volume":"22","author":"Lerner","year":"1996","journal-title":"Clin Infect Dis"},{"key":"R8","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S0934-8840(97)80092-8","article-title":"Identification of Borrelia burgdorferi sensu lato tick isolates from Slovakia by PCR typing with 16S rRNA primers","volume":"286","author":"Mateicka","year":"1997","journal-title":"Zentralbl Bakteriol"},{"key":"R9","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1128\/CMR.7.3.357","article-title":"The medically important aerobic actinomycetes: epidemiology and microbiology","volume":"7","author":"McNeil","year":"1994","journal-title":"Clin Microbiol Rev"},{"key":"R10","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1006\/abio.1996.9916","article-title":"Product differentiation by analysis of DNA melting curves during the polymerase chain reaction","volume":"245","author":"Ririe","year":"1997","journal-title":"Anal Biochem"},{"key":"R11","first-page":"1201","article-title":"Aerobic actinomycetes. 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