{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:43:52Z","timestamp":1762271032792},"reference-count":9,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,9,1]]},"abstract":"<jats:p>Following antigen preparation procedures similar to those of the direct agglutination test (DAT), an IgG ELISA employing intact <jats:italic>\u03b2<\/jats:italic>-mercaptoethanol (<jats:italic>\u03b2<\/jats:italic>-ME)-treated <jats:italic>Leishmania donovani<\/jats:italic> promastigotes was developed. The performance of the <jats:italic>\u03b2<\/jats:italic>-ME ELISA thus developed was assessed in patients with confirmed visceral leishmaniasis (VL), revealing slightly lower sensitivity (39\/40=97.5\u200a%) than that of the DAT (40\/40=100\u200a%). When challenged with sera of individuals with non-VL conditions, including leukaemia and African trypanosomiasis, the specificity of the <jats:italic>\u03b2<\/jats:italic>-ME ELISA was 100\u200a% (158\/158), compared to 98.8\u200a% (156\/158) for DAT. In an endemic population (<jats:italic>n<\/jats:italic>=145) manifesting a clinical suspicion of VL, results obtained with the <jats:italic>\u03b2<\/jats:italic>-ME ELISA were highly concordant with those of DAT, both in the seropositive (65\/68=95.6\u200a%) and seronegative (77\/80=96.3\u200a%) groups. Furthermore, the incorporated intact antigen demonstrated higher sensitivity in ELISA (16\/18=88.9\u200a%) than the water-soluble equivalent (13\/18=72.2\u200a%). The stability of the formaldehyde-fixed antigen (2\u2005months at 4\u200a\u00b0C) in <jats:italic>\u03b2<\/jats:italic>-ME ELISA, as well as the option for direct testing of whole-blood samples and visual reading of results (within 2\u2005h, compared to 18\u2005h for DAT), advocate the simultaneous application of the technique with DAT for confirmation of VL in laboratories with limited facilities.<\/jats:p>","DOI":"10.1099\/jmm.0.46643-0","type":"journal-article","created":{"date-parts":[[2006,8,16]],"date-time":"2006-08-16T18:42:50Z","timestamp":1155753770000},"page":"1193-1196","source":"Crossref","is-referenced-by-count":8,"title":["\u03b2-Mercaptoethanol-modified ELISA for diagnosis of visceral leishmaniasis"],"prefix":"10.1099","volume":"55","author":[{"given":"Elfadil M.","family":"Abass","sequence":"first","affiliation":[{"name":"Ahfad University for Women, PO Box 167, Omdurman, Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Durria","family":"Mansour","sequence":"additional","affiliation":[{"name":"Ahfad University for Women, PO Box 167, Omdurman, Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"el Mutasim","sequence":"additional","affiliation":[{"name":"Ahfad University for Women, PO Box 167, Omdurman, Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muna","family":"Hussein","sequence":"additional","affiliation":[{"name":"Omdurman Hospital for Tropical Diseases, Omdurman, Sudan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdallah","family":"el Harith","sequence":"additional","affiliation":[{"name":"Dutch Ministry of Foreign Affairs\/Mission to Ahfad University for Women, Omdurman, 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immunodiagnostic and epidemiological aspects","author":"Shiddo","year":"1995"},{"key":"R8","first-page":"359","article-title":"Enzyme-linked immunosorbent assay","volume-title":"In Manual of Clinical Immunology","author":"Voller","year":"1980"},{"key":"R9","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1046\/j.1365-3156.2001.00680.x","article-title":"Diagnosing visceral leishmaniasis with the recombinant K39 strip test: experience from Sudan","volume":"6","author":"Zijlstra","year":"2001","journal-title":"Trop Med Int Health"}],"container-title":["Journal of Medical 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