{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T14:31:35Z","timestamp":1781188295064,"version":"3.54.1"},"reference-count":47,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,2,1]]},"abstract":"<jats:p>Between January and September 2003, 39 isolates of the family <jats:italic>Enterobacteriaceae<\/jats:italic> with phenotypically positive Vitek 1 extended-spectrum beta-lactamase (ESBL) test results were collected, originating from patients of two hospitals in Saxony, Germany. Plasmid DNA was isolated and screened by PCR for the presence of genes encoding beta-lactamases of SHV, TEM and CTX-M types. To differentiate ESBL and non-ESBL among SHV and TEM genes, detailed analysis of PCR products was performed. Twenty-four strains carried SHV-2, SHV-5 or SHV-12 genes. In a further 11 strains a CTX-M gene was detected. The CTX-M genes could be affiliated to the CTX-M-1 and CTX-M-9 cluster by RFLP analysis. In the case of four <jats:italic>Klebsiella oxytoca<\/jats:italic> isolates, hyperproduction of the chromosomal beta-lactamase K1 was inferred, because genes of the above-mentioned types were not detected. The strains contained plasmid DNA between 45 and 160\u2005kb in size. Common plasmid restriction patterns among SHV-5 producers provided evidence of horizontal spread. Twenty strains had a MIC for cefotaxime of \u2a7d4\u2005mg\u2005l<jats:sup>\u22121<\/jats:sup>, 18 strains had the same MIC for ceftazidime, and nine strains had this MIC of &gt;4\u2005mg\u2005l<jats:sup>\u22121<\/jats:sup> for both antibiotics. The ESBL phenotypes often coincided with ciprofloxacin or gentamicin resistance.<\/jats:p>","DOI":"10.1099\/jmm.0.46670-0","type":"journal-article","created":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T21:03:47Z","timestamp":1169586227000},"page":"241-249","source":"Crossref","is-referenced-by-count":28,"title":["Molecular characterization of extended-spectrum beta-lactamases in Enterobacteriaceae from patients of two hospitals in Saxony, Germany"],"prefix":"10.1099","volume":"56","author":[{"given":"Joachim","family":"Schmitt","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Technical University of Dresden, Fetscherstrasse 72, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enno","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Technical University of Dresden, Fetscherstrasse 72, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Technical University of Dresden, Fetscherstrasse 72, Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1093\/jac\/dkg096","article-title":"CTX-M extended-spectrum \u03b2 -lactamase arrives in the UK","volume":"51","author":"Alobwede","year":"2003","journal-title":"J Antimicrob Chemother"},{"key":"R2","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1042\/bj2760269","article-title":"A standard numbering scheme for the class A \u03b2 -lactamases","volume":"276","author":"Ambler","year":"1991","journal-title":"Biochem J"},{"key":"R3","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1093\/jac\/45.2.183","article-title":"Antimicrobial resistance amongst 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