{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T10:46:17Z","timestamp":1789641977153,"version":"4.0.0"},"reference-count":18,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,9,1]]},"abstract":"<jats:p>At least eight species of <jats:italic>Cryptosporidium<\/jats:italic> can cause human infection and disease. A real-time PCR (qPCR) assay based on the 18S rRNA gene and utilizing a Scorpion probe was developed to detect all human-pathogenic <jats:italic>Cryptosporidium<\/jats:italic> without the usual need for nested amplification. Sensitivity of detection in stool samples was highest using a glass bead-based DNA extraction method (under 10<jats:sup>3<\/jats:sup> oocysts per stool sample). The assay was validated against 123 human stool specimens from Bangladesh and Tanzania, exhibited a sensitivity and specificity of &gt;91\u200a% versus microscopy, and detected an additional eight microscopy-negative infections. <jats:italic>Cryptosporidium parvum<\/jats:italic>-specific and <jats:italic>Cryptosporidium meleagridis<\/jats:italic>-specific Scorpion qPCR assays that provided 100\u200a% accurate speciation compared with <jats:italic>Vsp<\/jats:italic>I RFLP analysis and sequencing were developed subsequently. These Scorpion probe qPCR assays are simpler to perform than existing nested PCR and RFLP methods for diagnosis and epidemiological investigation of cryptosporidiosis.<\/jats:p>","DOI":"10.1099\/jmm.0.46678-0","type":"journal-article","created":{"date-parts":[[2006,8,16]],"date-time":"2006-08-16T18:42:50Z","timestamp":1155753770000},"page":"1217-1222","source":"Crossref","is-referenced-by-count":56,"title":["Real-time PCR detection and speciation of Cryptosporidium infection using Scorpion probes"],"prefix":"10.1099","volume":"55","author":[{"given":"Suzanne E.","family":"Stroup","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA 22908, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shantanu","family":"Roy","sequence":"additional","affiliation":[{"name":"Centre for Health and Population Research, International Centre for Diarrhoeal Disease Research, Dhaka-1000, 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