{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:07:59Z","timestamp":1791094079935,"version":"4.1.0"},"reference-count":29,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,10,1]]},"abstract":"<jats:p>Invasion of vascular endothelial cells is thought to be a critical step in the development of metastatic infections in patients with<jats:italic>Staphylococcus aureus<\/jats:italic>bacteraemia. This study was designed to evaluate the association between the ability to invade endothelial cells and metastatic infection by<jats:italic>S. aureus<\/jats:italic>. Patients with metastatic infection were identified among those with community-acquired<jats:italic>S. aureus<\/jats:italic>bacteraemia in a tertiary referral hospital. Patients with simple bacteraemia caused by<jats:italic>S. aureus<\/jats:italic>over the same period served as the control group. The ability of each clinical isolate to invade endothelial cells was evaluated by counting the number of intracellular organisms 1\u2005h after inoculation onto human umbilical vein endothelial cells<jats:italic>in vitro<\/jats:italic>. The cytotoxic activity of intracellular<jats:italic>S. aureus<\/jats:italic>was determined 24\u2005h after internalization, and expressed as the percentage of cells killed. The clinical isolates varied in invasiveness and cytotoxicity. The median invasiveness, relative to<jats:italic>S. aureus<\/jats:italic>reference strain ATCC 29213, was 145\u200a % in the cases (<jats:italic>n<\/jats:italic>=10) [interquartile range (IQR) 103\u2013160] and 153 \u200a% (IQR 111\u2013173) in the controls (<jats:italic>n<\/jats:italic>=11;<jats:italic>P<\/jats:italic>=0.44). The median cytotoxicity was 59.4 \u200a% (IQR 47\u201368) in the cases and 65.2 \u200a% (IQR 50\u201374) in the controls (<jats:italic>P<\/jats:italic>=0.44). Differences in the ability of<jats:italic>S. aureus<\/jats:italic>to invade and destroy vascular endothelial cells<jats:italic>in vitro<\/jats:italic>were not associated with the development of metastatic complications in patients with<jats:italic>S. aureus<\/jats:italic>bacteraemia. This implies that the invasiveness and toxicity of<jats:italic>S. aureus<\/jats:italic>for endothelial cells may not be major determinants of metastatic infection.<\/jats:p>","DOI":"10.1099\/jmm.0.46765-0","type":"journal-article","created":{"date-parts":[[2007,9,24]],"date-time":"2007-09-24T17:02:45Z","timestamp":1190653365000},"page":"1290-1295","source":"Crossref","is-referenced-by-count":4,"title":["In vitro ability of Staphylococcus aureus isolates from bacteraemic patients with and without metastatic complications to invade vascular endothelial cells"],"prefix":"10.1099","volume":"56","author":[{"given":"Wan Beom","family":"Park","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Han","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Seoul National University 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