{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T12:49:01Z","timestamp":1773751741120,"version":"3.50.1"},"reference-count":41,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,12,1]]},"abstract":"<jats:p>A prospective study was performed to evaluate the correlation between the proposed standard of the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antibiotic Susceptibility Testing (AFST-EUCAST) (document 7.1) and the commercial system Etest for determining the MICs of flucytosine, amphotericin B, fluconazole, itraconazole and voriconazole for a collection of 100 clinical and environmental isolates of <jats:italic>Cryptococcus neoformans<\/jats:italic>. The agreements among Etest MICs within \u00b12 log<jats:sub>2<\/jats:sub> dilutions of AFST-EUCAST standard MICs were greater for flucytosine, fluconazole and voriconazole (76, 78 and 88\u200a%, respectively) than for amphotericin B (5\u200a%), the lowest agreement, and itraconazole (67\u200a%). Overall, the correlation coefficients were statistically significant (<jats:italic>P<\/jats:italic>&lt;0.05), and it is suggested that the Etest and AFST-EUCAST method are reliable alternatives and present good correlation for all drugs evaluated except amphotericin B. However, the observed differences related to MICs for susceptible, susceptible dose dependent and resistant strains between the methods suggest that it will be necessary to carry out further studies, including assessment of interlaboratory agreement and correlation of MICs by different methods with <jats:italic>in vivo<\/jats:italic> response.<\/jats:p>","DOI":"10.1099\/jmm.0.46789-0","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T03:24:42Z","timestamp":1163647482000},"page":"1693-1699","source":"Crossref","is-referenced-by-count":18,"title":["Comparative analysis of Etest and broth microdilution method (AFST-EUCAST) for trends in antifungal drug susceptibility testing of Brazilian Cryptococcus neoformans isolates"],"prefix":"10.1099","volume":"55","author":[{"given":"Amanda L. T.","family":"Dias","sequence":"first","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas, Laborat\u00f3rio de Microbiologia, Universidade Federal de Alfenas-MG, Alfenas-MG, Brazil"},{"name":"Departamento de Microbiologia, Laborat\u00f3rio de Micologia, Instituto de Ci\u00eancias Biom\u00e9dicas II, Universidade de S\u00e3o Paulo (USP), S\u00e3o Paulo-SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavia E.","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Laborat\u00f3rio de Micologia, Instituto de Ci\u00eancias Biom\u00e9dicas II, Universidade de S\u00e3o Paulo (USP), S\u00e3o Paulo-SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcia S. 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