{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:48:10Z","timestamp":1762865290459},"reference-count":41,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,6,1]]},"abstract":"<jats:p>Infections by <jats:italic>Shigella<\/jats:italic> species are an important cause of diarrhoeal disease worldwide. Of 4198 <jats:italic>Shigella<\/jats:italic> isolates received by the French National Reference Centre for <jats:italic>Escherichia coli<\/jats:italic> and <jats:italic>Shigella<\/jats:italic>, 180 from patients with diarrhoea and dysentery in 2000\u20132004 did not react with any available polyclonal rabbit antisera used to identify the established <jats:italic>Shigella<\/jats:italic> serogroups. This study describes the molecular and phenotypic characteristics of these isolates in seroagglutination tests, molecular serotyping (<jats:italic>rfb<\/jats:italic>-RFLP and <jats:italic>fliC<\/jats:italic>-RFLP), ribotyping, detection of invasivity and enterotoxins genes, and antibiotic sensitivity. All isolates gave biochemical reactions typical of <jats:italic>Shigella boydii<\/jats:italic>, were mannitol-positive and indole-negative. They all carried invasion-associated genes, enterotoxin 2 [ShET-2] and an IS<jats:italic>630<\/jats:italic> sequence. They had a unique ribotype that was distinct from all other <jats:italic>Shigella<\/jats:italic> and <jats:italic>E. coli<\/jats:italic> patterns. Further characterization by <jats:italic>rfb<\/jats:italic>-RFLP clearly distinguished this serogroup from all other <jats:italic>Shigella<\/jats:italic> or <jats:italic>E. coli<\/jats:italic> O-groups. The <jats:italic>fliC<\/jats:italic>-RFLP pattern corresponded to P4, an F-pattern which is associated with 10 different serogroups of <jats:italic>S. boydii<\/jats:italic>. A new antiserum prepared against strain 00-977 agglutinated all 180 isolates and cross-agglutination and absorption studies with anti-00-977 serum and anti-CDC 99-4528 (reference for the newly described <jats:italic>S. boydii<\/jats:italic> serogroup 20) serum showed identical antigenic structure. Furthermore, strains 00-977 and CDC 99-4528 had the same molecular serotype, ribotype and virulence genes.<\/jats:p>","DOI":"10.1099\/jmm.0.46818-0","type":"journal-article","created":{"date-parts":[[2007,5,17]],"date-time":"2007-05-17T22:41:59Z","timestamp":1179441719000},"page":"749-754","source":"Crossref","is-referenced-by-count":26,"title":["Identification of a group of shigella-like isolates as Shigella boydii 20"],"prefix":"10.1099","volume":"56","author":[{"given":"Francine","family":"Grimont","sequence":"first","affiliation":[{"name":"French National Reference Centre for Escherichia coli and Shigella, Unit\u00e9 de Biodiversit\u00e9 des Bact\u00e9ries Pathog\u00e8nes Emergentes, Institut Pasteur, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique","family":"Lejay-Collin","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Escherichia coli and Shigella, Unit\u00e9 de Biodiversit\u00e9 des Bact\u00e9ries Pathog\u00e8nes Emergentes, Institut Pasteur, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaisar A.","family":"Talukder","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Diseases Research, Dhaka-100, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Carle","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Escherichia coli and Shigella, Unit\u00e9 de Biodiversit\u00e9 des Bact\u00e9ries Pathog\u00e8nes Emergentes, Institut Pasteur, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Issenhuth","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Escherichia coli and Shigella, Unit\u00e9 de Biodiversit\u00e9 des Bact\u00e9ries Pathog\u00e8nes Emergentes, Institut Pasteur, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine","family":"Le Roux","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Escherichia coli and Shigella, Unit\u00e9 de Biodiversit\u00e9 des Bact\u00e9ries Pathog\u00e8nes Emergentes, Institut Pasteur, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick A. 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