{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:39:50Z","timestamp":1777491590786,"version":"3.51.4"},"reference-count":20,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,7,1]]},"abstract":"<jats:p>This study evaluated a novel peptide nucleic acid (PNA) probe targeting a region of the 23S rRNA gene of <jats:italic>Klebsiella pneumoniae<\/jats:italic> by fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH). Analytical performance was determined using 39 reference strains and other well-characterized strains of <jats:italic>Klebsiella<\/jats:italic> spp. and <jats:italic>Enterobacter aerogenes<\/jats:italic>. The probe was found to be specific for the <jats:italic>K. pneumoniae<\/jats:italic> complex (<jats:italic>K. pneumoniae<\/jats:italic> including <jats:italic>Klebsiella ozaenae<\/jats:italic> and <jats:italic>Klebsiella variicola<\/jats:italic>). The diagnostic accuracy was evaluated with 264 blood cultures containing Gram-negative rods. Using conventional identification as the reference, performance specifications were as follows: sensitivity 98.8\u200a%, specificity 99.5\u200a%, positive predictive value 98.8\u200a% and negative predictive value 99.5\u200a%. Discrepancies were resolved by PNA FISH retest and phenotypic tests. In conclusion, the <jats:italic>K. pneumoniae<\/jats:italic> probe provided an accurate diagnosis within 3 h and may supplement other methods for direct identification of Gram-negative bacteria.<\/jats:p>","DOI":"10.1099\/jmm.0.46829-0","type":"journal-article","created":{"date-parts":[[2007,6,18]],"date-time":"2007-06-18T22:42:55Z","timestamp":1182206575000},"page":"914-917","source":"Crossref","is-referenced-by-count":29,"title":["Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood cultures"],"prefix":"10.1099","volume":"56","author":[{"given":"Mette","family":"S\u00f8gaard","sequence":"first","affiliation":[{"name":"Department of Clinical Epidemiology, Aalborg Hospital, Aarhus University Hospital, Aarhus, Denmark"},{"name":"Department of Clinical Microbiology, Aalborg Hospital, Aarhus University Hospital, Postbox 365, 9100 Aalborg, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis 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