{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:50:28Z","timestamp":1790707828554,"version":"4.1.0"},"reference-count":12,"publisher":"Microbiology Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,1,1]]},"abstract":"The prevalence of CTX-M-producing members of the Enterobacteriaceae<\/jats:italic> is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla<\/jats:italic>\n CTX-M<\/jats:sub> genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on \u03b2<\/jats:italic>-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae<\/jats:italic> isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum \u03b2<\/jats:italic>-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla<\/jats:italic>\n CTX-M<\/jats:sub> genes in 21 of 28 ESBL-producing isolates.<\/jats:p>","DOI":"10.1099\/jmm.0.46909-0","type":"journal-article","created":{"date-parts":[[2006,12,15]],"date-time":"2006-12-15T19:51:25Z","timestamp":1166212285000},"page":"52-55","source":"Crossref","is-referenced-by-count":126,"title":["Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum \u03b2-lactamases"],"prefix":"10.1099","volume":"56","author":[{"given":"Christopher I.","family":"Birkett","sequence":"first","affiliation":[{"name":"Health Protection Agency, Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo A.","family":"Ludlam","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Woodford","sequence":"additional","affiliation":[{"name":"Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek F. 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