{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T01:43:24Z","timestamp":1775180604711,"version":"3.50.1"},"reference-count":16,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,4,1]]},"abstract":"<jats:p>Isolation of<jats:italic>Neisseria gonorrhoeae<\/jats:italic>is currently the gold standard for the definitive diagnosis of gonorrhoea and for use in medico-legal cases in the UK. Molecular detection methods are used increasingly but are untested as evidence of infection in a court of law. An isolate of<jats:italic>N. gonorrhoeae<\/jats:italic>was obtained from a child and an article of clothing from an adult male who was suspected of sexual abuse of the child. Biochemical and immunological tests were used to confirm the isolate as<jats:italic>N. gonorrhoeae<\/jats:italic>. Amplification by PCR using two targets,<jats:italic>cppB<\/jats:italic>and<jats:italic>omp<\/jats:italic>III, was used both as further confirmation of the isolate and to detect the presence of gonococcal-specific DNA from the clothing. The relationship of the gonococcal DNA from the child and the adult was investigated using genotyping (<jats:italic>N. gonorrhoeae<\/jats:italic>multi-antigen sequence typing; NG-MAST), including a nested PCR for the<jats:italic>por<\/jats:italic>gene. Both samples were indistinguishable by NG-MAST and shared the same sequence type, 403. This is the first report of molecular detection and genotyping of<jats:italic>N. gonorrhoeae<\/jats:italic>on an article of clothing, which resulted in conviction of the man for sexual assault.<\/jats:p>","DOI":"10.1099\/jmm.0.46956-0","type":"journal-article","created":{"date-parts":[[2007,3,21]],"date-time":"2007-03-21T00:27:12Z","timestamp":1174436832000},"page":"487-490","source":"Crossref","is-referenced-by-count":20,"title":["Non-cultural detection and molecular genotyping of Neisseria gonorrhoeae from a piece of clothing"],"prefix":"10.1099","volume":"56","author":[{"given":"Iona M. 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