{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:05:58Z","timestamp":1789985158056,"version":"4.0.1"},"reference-count":33,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,8,1]]},"abstract":"<jats:p>\n                  <jats:italic>Burkholderia pseudomallei<\/jats:italic> is the causative agent of melioidosis, an infectious disease of humans and animals. Gene clusters which encode capsular polysaccharide (type I O-PS) and LPS (type II O-PS), both of which play roles in virulence, have previously been identified. Here, the identification of two further putative clusters, type III O-PS and type IV O-PS, is reported. Mice challenged with type III O-PS or type IV O-PS mutants showed increased mean times to death (7.8 and 11.6 days) compared to those challenged with wild-type <jats:italic>B. pseudomallei<\/jats:italic> (3 days). To investigate the possible roles of polysaccharides in protection, mice were immunized with killed cells of wild-type <jats:italic>B. pseudomallei<\/jats:italic> or killed cells of <jats:italic>B. pseudomallei<\/jats:italic> with mutations in the O antigen, capsular polysaccharide, type III O-PS or type IV O-PS gene clusters. Immunization with all polysaccharide mutant strains resulted in delayed time to death compared to the na\u00efve controls, following challenge with wild-type <jats:italic>B. pseudomallei<\/jats:italic> strain K96243. However, immunization with killed polysaccharide mutant strains conferred different degrees of protection, demonstrating the immunological importance of the polysaccharide clusters on the surface of <jats:italic>B. pseudomallei<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/jmm.0.47043-0","type":"journal-article","created":{"date-parts":[[2007,7,20]],"date-time":"2007-07-20T17:52:59Z","timestamp":1184953979000},"page":"1005-1010","source":"Crossref","is-referenced-by-count":44,"title":["Polysaccharides and virulence of Burkholderia pseudomallei"],"prefix":"10.1099","volume":"56","author":[{"given":"M.","family":"Sarkar-Tyson","sequence":"first","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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